PRODUCT CODE: EM1701-52

HNP-1 Mouse Monoclonal Antibody [2D2] (EM1701-52)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

Western blot analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.
  • Western blot analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HNP-1 antibody. Counter stained with hematoxylin.
Western blot analysis of HNP-1 on rat spleen tissue lysates using anti-HNP-1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HNP-1 Mouse Monoclonal Antibody [2D2] (EM1701-52)

Immunogen

Synthetic peptide within human hnp-1 aa 1-50 / 94.

Host

Mouse

Positive Control

Rat spleen tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2D2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

10 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

HNP-1

SYNONYMS

alpha 1 antibody; DEF1 antibody; DEF1_HUMAN antibody; DEFA1 antibody; DEFA1B antibody; DEFA2 antibody; Defensin 1 antibody; Defensin antibody; Defensin, alpha 1 antibody; Defensin, alpha 1, myeloid related sequence antibody; Defensin, alpha 2 antibody; HNP-1 antibody; HNP-2 antibody; HNP1 antibody; HP-1 antibody; HP-2 antibody; HP1 antibody; HP2 antibody; MRS antibody; Myeloid related sequence antibody; Neutrophil defensin 1 antibody; Neutrophil defensin 2 antibody

SEQUENCE SIMILARITIES

Belongs to the alpha-defensin family.

POST-TRANSLATIONAL MODIFICATION

ADP-ribosylation drastically reduces cytotoxic and antibacterial activities, and enhances IL8 production.; Phosphorylation at Tyr-85 has been found in some cancer cell lines, and interferes with ADP-ribosylation.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation.