Positive control:
Lane 1: A431 cell lysate
Lane 2: Hela cell lysate
Lane 3: Mouse stomach tissue lysate
Lane 4: Rat cerebellum tissue lysate
Applications
-
WB
-
IHC
-
FC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
hNaa50p Rabbit Polyclonal Antibody (ER2001-66)
Immunogen
Synthetic peptide within human hnaa50p aa 1-50.
Host
Rabbit
Positive Control
A431 cell lysate, Hela cell lysate, mouse stomach tissue lysate, rat cerebellum tissue lysate, rat brain tissue, human thyroid tissue, rat cerebellum tissue, human skin tissue, human breast carcinoma tissue, Daudi.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Peptide affinity purified.
MOLECULAR WEIGHT
Predicted band size: 19/9 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
IHC
-
1:200-1:1,000
-
FC
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
hNaa50p
SYNONYMS
MAK3 antibody; FLJ13194 antibody; hNAT5 antibody; hSAN antibody; Mak3 homolog (S. cerevisiae) antibody; Mak3 homolog antibody; N-acetyltransferase 13 (GCN5-related) antibody; N-acetyltransferase 13 antibody; N-acetyltransferase 5 antibody; N-acetyltransferase san homolog antibody; N-alpha-acetyltransferase 50 antibody; N-alpha-acetyltransferase 50, NatE catalytic subunit antibody; Naa50 antibody; NAA50_HUMAN antibody; NAT 5 antibody; NAT13 antibody; NAT5 antibody; NatE catalytic subunit antibody; San antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
N-alpha-acetyltransferase that acetylates the N-terminus of proteins that retain their initiating methionine . Has a broad substrate specificity: able to acetylate the initiator methionine of most peptides, except for those with a proline in second position . Also displays N-epsilon-acetyltransferase activity by mediating acetylation of the side chain of specific lysines on proteins . Autoacetylates in vivo . The relevance of N-epsilon-acetyltransferase activity is however unclear: able to acetylate H4 in vitro, but this result has not been confirmed in vivo . Component of a N-alpha-acetyltransferase complex containing NAA10 and NAA15, but NAA50 does not influence the acetyltransferase activity of NAA10: this multiprotein complex probably constitutes the major contributor for N-terminal acetylation at the ribosome exit tunnel, with NAA10 acetylating all amino termini that are devoid of methionine and NAA50 acetylating other peptides . Required for sister chromatid cohesion during mitosis by promoting binding of CDCA5/sororin to cohesin: may act by counteracting the function of NAA10 .