HLA-DQA1 Recombinant Rabbit Monoclonal Antibody [JU17-34] (ET1706-51)
Catalog# ET1706-51
Rabbit Monoclonal to HLA-DQA1
Tested for
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WB
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IHC-P
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IP
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IF-Cell
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mIHC
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HLA-DQA1 on different lysates with Rabbit anti-HLA-DQA1 antibody (ET1706-51) at 1/500 dilution.
Lane 1: Rat lung tissue lysate, 20 µg/Lane
Lane 2: Rat skin tissue lysate, 20 µg/Lane
Lane 3: Mouse thymus tissue lysate, 20 µg/Lane
Lane 4: Mouse spleen tissue lysate, 20 µg/Lane
Lane 5: Raji cell lysate, 10 µg/Lane
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-51) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.
Lane 1: Rat lung tissue lysate, 20 µg/Lane
Lane 2: Rat skin tissue lysate, 20 µg/Lane
Lane 3: Mouse thymus tissue lysate, 20 µg/Lane
Lane 4: Mouse spleen tissue lysate, 20 µg/Lane
Lane 5: Raji cell lysate, 10 µg/Lane
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 2 minutes;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-51) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 200,000 dilution was used for 1 hour at room temperature.