PRODUCT CODE: EM1801-09

HLA Class 1 ABC Mouse Monoclonal Antibody [11C3] (EM1801-09)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

Western blot analysis of HLA Class 1 ABC on Hela lysate. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal ECL Western Blotting Substrate (Product # K1801).
  • Western blot analysis of HLA Class 1 ABC on Hela lysate. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal ECL Western Blotting Substrate (Product # K1801).
  • Immunocytochemistry staining HLA Class 1 ABC in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with Ki67 polyclonal antibody at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Mouse IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of HLA Class 1 ABC on Hela lysate. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hour at room temperature. Chemiluminescent detection was performed using SuperSignal ECL Western Blotting Substrate (Product # K1801).

Applications

  • WB

  • ICC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HLA Class 1 ABC Mouse Monoclonal Antibody [11C3] (EM1801-09)

Immunogen

Recombinant protein within human hla class 1 abc aa 120-310.

Host

Mouse

Positive Control

Hela, 293T.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

11C3

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB:1:500-1:2000

  • ICC:1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

HLA Class 1 ABC

SYNONYMS

Antigen presenting molecule antibody; HLA class I histocompatibility antigen, A 1 alpha chain antibody; HLAA antibody; Leukocyte antigen class I A antibody; Major histocompatibility complex, class I, A antibody; MHC class I antigen HLA A heavy chain antibody; AS antibody; HLA B protein antibody; HLA class I histocompatibility antigen, B alpha chain antibody; HLA-B antibody; HLA-B histocompatibility type antibody; HLAB antibody; leukocyte antigen class I-B antibody; lymphocyte antigen antibody; major histocompatibility complex, class I, B antibody; MHC class I antigen GN00104 antibody; MHC class I antigen HLA-B heavy chain antibody; MHC class I antigen SHCHA antibody; MHC Class I HLA heavy chain antibody; MHC HLA-B cell surface glycoprotein antibody; MHC HLA-B transmembrane glycoprotein antibody; SPDA1 antibody; 1C07_HUMAN antibody; Cw-7 alpha chain antibody; D6S204 antibody; HLA class I histocompatibility antigen antibody; HLA class I histocompatibility antigen, C alpha chain antibody; HLA class I histocompatibility antigen, Cw-1 alpha chain antibody; HLA JY3 antibody; HLA-C antibody; HLA-C histocompatibility type antibody; HLA-Cw antibody; HLA-JY3 antibody; HLAC antibody; HLC C antibody; HLC-C antibody; human leukocyte antigen-C alpha chain antibody; major histocompatibility antigen HLA-C antibody; major histocompatibility complex, class I, C antibody; MHC class I antigen antibody; MHC class I antigen Cw 1 antibody; MHC class I antigen Cw*7 antibody; MHC class I antigen heavy chain HLA-C antibody; PSORS1 antibody

SUBCELLULAR LOCATION

Cell Membrane.

FUNCTION

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described.