Histone H3 (mutated K27 M) Recombinant Rabbit Monoclonal Antibody [JE46-51] (HA722200)
Catalog# HA722200
Rabbit Monoclonal to Histone H3 (mutated K27 M)
Tested for
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WB
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IF-Cell
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IHC-P
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ChIP
Reacts with
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Human
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Mouse
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Histone H3 (mutated K27 M) on different lysates with Rabbit anti-Histone H3 (mutated K27 M) antibody (HA722200) at 1/1,000 dilution.
Lane 1: 293T-OE-Histone H3 (mutated K27 M) cell lysate
Lane 2: 293T-OE-Histone H3 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 18 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T-OE-Histone H3 (mutated K27 M) cell lysate
Lane 2: 293T-OE-Histone H3 cell lysate (negative)
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 18 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722200) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.