Histone H3 Rabbit Polyclonal Antibody (HA500298)
Catalog# HA500298
Rabbit Polyclonal to Histone H3
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WB
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Zebrafish
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Human
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Mouse
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 15 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500298) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Histone H3 Rabbit Polyclonal Antibody (HA500298)
Immunogen
Synthetic peptide within zebrafish histone h3 aa 1-50 / 136.
Host
Rabbit
Positive Control
Zebrafish tissue lysates.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:5,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 15 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Histone H3
SYNONYMS
H3 histone family member E pseudogene antibody;H3 histone family, member A antibody;H3/A antibody;H31_HUMAN antibody;H3F3 antibody;H3FA antibody;Hist1h3a antibody;HIST1H3B antibody;HIST1H3C antibody;HIST1H3D antibody;HIST1H3E antibody;HIST1H3F antibody;HIST1H3G antibody;HIST1H3H antibody;HIST1H3I antibody;HIST1H3J antibody;HIST3H3 antibody;histone 1, H3a antibody;Histone cluster 1, H3a antibody;Histone H3 3 pseudogene antibody;Histone H3.1 antibody;Histone H3/a antibody;Histone H3/b antibody;Histone H3/c antibody;Histone H3/d antibody;Histone H3/f antibody;Histone H3/h antibody;Histone H3/i antibody;Histone H3/j antibody;Histone H3/k antibody;Histone H3/l antibody
SUBCELLULAR LOCATION
Nucleus,Chromosome.
FUNCTION
Variant histone H3 which replaces conventional H3 in a wide range of nucleosomes in active genes. Constitutes the predominant form of histone H3 in non-dividing cells and is incorporated into chromatin independently of DNA synthesis. Deposited at sites of nucleosomal displacement throughout transcribed genes, suggesting that it represents an epigenetic imprint of transcriptionally active chromatin. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
CITATIONS
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Luan Yi,et al
Linear ubiquitination regulates the KSHV replication and transcription activator protein to control infection
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WB
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Human
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Citation
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