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Histone H3 Mouse Monoclonal Antibody [A11-D7] (M1309-1)

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Catalog# M1309-1

Mouse Monoclonal to Histone H3

Tested for

  • WB

  • IF-Cell

  • IHC-P

  • IF-Tissue

Reacts with

  • Human

  • Mouse

  • Rat

  • Zebrafish

This product has been cited in peer reviewed publications, see list HERE

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Specifications

Product Type

Mouse monoclonal primary

Product Name

Histone H3 Mouse Monoclonal Antibody [A11-D7] (M1309-1)

Immunogen

Synthetic peptide within n-terminal human histone h3.

Host

Mouse

Positive Control

HeLa cell lysate, A549 cell lysate, HT-29 cell lysate, HEK-293 cell lysate, C2C12 cell lysate, L-929 cell lysate, C6 cell lysate, zebrafish tissue lysates, HepG2 cell lysate, A431 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Hela, F9, human skin tissue, human liver tissue, human testis tissue, mouse brain tissue, mouse testis tissue, rat testis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A11-D7

RRID

AB_3073064

APPLICATION DILUTION

  • WB

  • 1:10,000-1:20,000

  • IF-Cell

  • 1:200-1:500

  • IHC-P

  • 1:1,000

  • IF-Tissue

  • 1:200

PROPERTIES

Form

Liquid

Storage Condition

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

Purification

Protein A affinity purified.

Molecular Weight

Predicted band size: 15 kDa

Isotype

IgG2b

TARGET

UNIPROT #

P68431

PROTEIN NAME

Histone H3

SYNONYMS

H3 histone family member E pseudogene antibody;H3 histone family;member A antibody;H3/A antibody;H31_HUMAN antibody;H3F3 antibody;H3FA antibody;Hist1h3a antibody;HIST1H3B antibody;HIST1H3C antibody;HIST1H3D antibody;HIST1H3E antibody;HIST1H3F antibody;HIST1H3G antibody;HIST1H3H antibody;HIST1H3I antibody;HIST1H3J antibody;HIST3H3 antibody;histone 1;H3a antibody;Histone cluster 1;H3a antibody;Histone H3 3 pseudogene antibody;Histone H3.1 antibody;Histone H3/a antibody;Histone H3/b antibody;Histone H3/c antibody;Histone H3/d antibody;Histone H3/f antibody;Histone H3/h antibody;Histone H3/i antibody;Histone H3/j antibody;Histone H3/k antibody;Histone H3/l antibody

SEQUENCE SIMILARITIES

Belongs to the histone H3 family.

DEVELOPMENTAL STAGE

Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.

POST-TRANSLATIONAL MODIFICATION

Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me). Acetylation at Lys-123 (H3K122ac) by EP300/p300 plays a central role in chromatin structure: localizes at the surface of the histone octamer and stimulates transcription, possibly by promoting nucleosome instability.; Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.; Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.; Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin. Monomethylation at Lys-57 (H3K56me1) by EHMT2/G9A in G1 phase promotes interaction with PCNA and is required for DNA replication.; Phosphorylated at Thr-4 (H3T3ph) by HASPIN during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MAP3K20 isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Thr-12 (H3T11ph) by chromatin-associated CHEK1 regulates the transcription of cell cycle regulatory genes by modulating acetylation of Lys-10 (H3K9ac). Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.; Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.; Lysine deamination at Lys-5 (H3K4all) to form allysine is mediated by LOXL2. Allysine formation by LOXL2 only takes place on H3K4me3 and results in gene repression.; Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.; Butyrylation of histones marks active promoters and competes with histone acetylation. It is present during late spermatogenesis.; Succinylation at Lys-80 (H3K79succ) by KAT2A takes place with a maximum frequency around the transcription start sites of genes. It gives a specific tag for epigenetic transcription activation. Desuccinylation at Lys-123 (H3K122succ) by SIRT7 in response to DNA damage promotes chromatin condensation and double-strand breaks (DSBs) repair.; Serine ADP-ribosylation constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Serine ADP-ribosylation at Ser-11 (H3S10ADPr) is mutually exclusive with phosphorylation at Ser-11 (H3S10ph) and impairs acetylation at Lys-10 (H3K9ac).

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

CITATIONS

  • Tang, G., Yuan, J., Wang, J., Zhang, Y. Z., Xie, S. S., Wang, H., Tao, Z., Liu, H., Kistler, H. C., Zhao, Y., Duan, C. G., Liu, W., Ma, Z., & Chen, Y.

    Fusarium BP1 is a reader of H3K27 methylation

  • Song Yangran,et al

    Histone H1. 2 Inhibited EMCV Replication through Enhancing MDA5-Mediated IFN-β Signaling Pathway

  • Destruction of self-derived PAMP via T3SS2 effector VopY to subvert PAMP-triggered immunity mediates Vibrio parahaemolyticus pathogenicity

  • Jiwen L;Jiemin Wong

    SUMO suppresses and MYC amplifies transcription globally by regulating CDK9 sumoylation

  • Wen, H., Shi, H., Jiang, N., Qiu, J., Lin, F., & Kou, Y.

    Antifungal mechanisms of silver nanoparticles on mycotoxin producing rice false smut fungus

  • Meng, S., Shi, H., Lin, C., Wu, Z., Lin, F., Tao, Z., & Kou, Y.

    UvKmt2-Mediated H3K4 Trimethylation Is Required for Pathogenicity and Stress Response in Ustilaginoidea virens

  • Zou Chao-Chun

    Vitamin C deficiency aggravates tumor necrosis factor α-induced insulin resistance

  • Tong Haojie,et al

    Essential roles of histone lysine methyltransferases EZH2 and EHMT1 in male embryo development of Phenacoccus solenopsis

  • Chuan Sun,et al

    Insufficient Effective Time of Suberanilohydroxamic Acid, a Deacetylase Inhibitor, Treatment Promotes PC3 Cell Growth

  • Xu Y, Qu M, He Y, et al

    Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions

  • Ting Liu,et al

    Dynamic control of RNA-DNA hybrid formation orchestrates DNA2 activation at stalled forks by RNAPII and DDX39A

  • Xu Mengting,et al

    A repressive H3K36me2 reader mediates Polycomb silencing

  • Meng, S., Liu, Z., Shi, H., Wu, Z., Qiu, J., Wen, H., Lin, F., Tao, Z., Luo, C., & Kou, Y.

    UvKmt6-mediated H3K27 trimethylation is required for development, pathogenicity, and stress response in Ustilaginoidea virens

  • Geng, Q., Li, H., Wang, D., Sheng, R. C., Zhu, H., Klosterman, S. J., Subbarao, K. V., Chen, J. Y., Chen, F. M., & Zhang, D. D.

    The Verticillium dahliae Spt-Ada-Gcn5 Acetyltransferase Complex Subunit Ada1 Is Essential for Conidia and Microsclerotia Production and Contributes to Virulence

  • Tandem mass tag‐based proteomic analysis of endoplasmic reticulum proteins in mulberry leaves under ultraviolet‐B and dark stress

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