Acetyl-Histone H3 (K18) Mouse Monoclonal Antibody [A7B5] (HA600090)
Catalog# HA600090
Mouse Monoclonal to Acetyl-Histone H3 (K18)
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Rat
Western blot analysis of Histone H3 (acetyl K18) on different lysates with Mouse anti-Histone H3 (acetyl K18) antibody (HA600090) at 1/2,000 dilution.
Lane 1: Hela cell lysate
Lane 2: Hela treated with TSA cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 14/40 kDa
Exposure time: 30 seconds;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Lane 1: Hela cell lysate
Lane 2: Hela treated with TSA cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 14/40 kDa
Exposure time: 30 seconds;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA600090) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
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