PRODUCT CODE: R1601-7

Histone Deacetylase 2 Rabbit Polyclonal Antibody (R1601-7)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: SH-SY5Y<br />
   Lane 2: 293T<br />
 Lane 3: Hela <br />
     Lane 4: PC-12
  • Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution.<br />
 Positive control:<br />
 Lane 1: SH-SY5Y<br />
   Lane 2: 293T<br />
 Lane 3: Hela <br />
     Lane 4: PC-12
  • ICC staining HDAC2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC2 in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HDAC2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of SH-SY5Y cells with HDAC2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of HDAC2 on different cell lysates using anti-HDAC2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: SH-SY5Y
Lane 2: 293T
Lane 3: Hela
Lane 4: PC-12

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Histone Deacetylase 2 Rabbit Polyclonal Antibody (R1601-7)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

SH-SY5Y, 293T, Hela, PC-12, LOVO, NIH-3T3, human tonsil tissue, human colon cancer tissue, human kidney tissue, mouse brain tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

55 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:500-1:2,000

  • IHC-P

  • 1:100-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Histone Deacetylase 2

SYNONYMS

D10Wsu179e antibody; HD 2 antibody; HD2 antibody; HDAC 2 antibody; Hdac2 antibody; HDAC2_HUMAN antibody; Histone deacetylase 2 (HD2) antibody; Histone deacetylase 2 antibody; OTTHUMP00000017046 antibody; OTTHUMP00000227077 antibody; OTTHUMP00000227078 antibody; RPD3 antibody; transcriptional regulator homolog RPD3 antibody; YAF1 antibody; YY1 associated factor 1 antibody; YY1 transcription factor binding protein antibody; Yy1bp antibody

SEQUENCE SIMILARITIES

Belongs to the histone deacetylase family. HD type 1 subfamily.

TISSUE SPECIFICITY

Widely expressed; lower levels in brain and lung.

POST-TRANSLATIONAL MODIFICATION

S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

SUBCELLULAR LOCATION

Nucleus. Cytoplasm.

FUNCTION

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation, and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.