PRODUCT CODE: 0812-1

His-Tag Rabbit Polyclonal Antibody (0812-1)

Applications

  • WB

REACTIVITY

Western blot analysis of His-Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0812-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: C-terminal His-tagged recombinant protein<br />
Lane 2: N-terminal His-tagged recombinant protein
  • Western blot analysis of His-Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0812-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: C-terminal His-tagged recombinant protein<br />
Lane 2: N-terminal His-tagged recombinant protein
Western blot analysis of His-Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0812-1, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal His-tagged recombinant protein
Lane 2: N-terminal His-tagged recombinant protein

Applications

  • WB

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

His-Tag Rabbit Polyclonal Antibody (0812-1)

Immunogen

Synthetic peptide immune sequence is hhhhhhc.

Host

Rabbit

Positive Control

C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2000

TARGET

PROTEIN NAME

His-Tag

FUNCTION

His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.