6X His tag Rabbit Polyclonal Antibody (0812-1)
Catalog# 0812-1
Rabbit Polyclonal to 6X His tag
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WB
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IP
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Species independent
Positive control:
Lane 1: C-terminal His-tagged recombinant protein
Lane 2: N-terminal His-tagged recombinant protein
Specifications
Product Type
Rabbit polyclonal primary
Product Name
6X His tag Rabbit Polyclonal Antibody (0812-1)
Immunogen
Synthetic peptide immune sequence is hhhhhhc.
Host
Rabbit
Positive Control
C-terminal His-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:2000
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IP
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2-5 µg/ml.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
6X His tag
FUNCTION
His-Tag Antibody detects recombinant proteins containing the 6xHis epitope tag. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
CITATIONS
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Cell-free protein synthesis of CD1E and B2M protein and in vitro interaction
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WB
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Citation
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MrSt12 implicated in the regulation of transcription factor AFTF1 by Fus3-MAPK during cuticle penetration by the entomopathogenic fungus Metarhizium robertsii.
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WB
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E. coli
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Citation
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Characterization of CD3γ/δ+ cells in grass carp (Ctenopharyngodon idella)
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WB
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Mouse
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Citation
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Dual RNA Sequencing of Beauveria bassiana-Infected Spodoptera frugiperda Reveals a Fungal Protease with Entomopathogenic and Antiphytopathogenic Activities
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WB
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E. coli
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Citation
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The evolution and functional characterization of CXC chemokines and receptors in lamprey
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WB
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Human
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Citation
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Xu Y, Qu M, He Y, et al
Smurf1 polyubiquitinates on K285/K282 of the kinases Mst1/2 to attenuate their tumor-suppressor functions
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WB
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Human
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Mouse
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Citation
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-
TRIM21 restricts influenza A virus replication by ubiquitination-dependent degradation of M1
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WB
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Human
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Citation
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