HFE Recombinant Rabbit Monoclonal Antibody [JE58-66] (HA720039)
Catalog# HA720039
Rabbit Monoclonal to HFE
Tested for
-
WB
Reacts with
-
Human
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HFE on different lysates with Rabbit anti-HFE antibody (HA720039) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A375 cell lysate
Lane 3: Caco-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720039) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A375 cell lysate
Lane 3: Caco-2 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA720039) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.