Hexokinase II Rabbit Polyclonal Antibody (HA500186)
Catalog# HA500186
Rabbit Polyclonal to Hexokinase II
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WB
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IF-Cell
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: 293 cell lysate (10 µg/Lane)
Lane 2: Jurkat cell lysate (10 µg/Lane)
Lane 3: PC-12 cell lysate (10 µg/Lane)
Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
Lane 5: Rat skeletal muscle tissue lysate (20 µg/Lane)
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 2 minutes;
8% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500186) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
Hexokinase II Rabbit Polyclonal Antibody (HA500186)
Immunogen
Recombinant protein within human hexokinase ii aa 1-200.
Host
Rabbit
Positive Control
293 cell lysate, Jurkat cell lysate, PC-12 cell lysate, NIH/3T3 cell lysate, rat skeletal muscle tissue lysate, SiHa, rat testis tissue, human fetal skeletal muscle tissue, mouse kidney tissue, Hela, PC-12.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:50-1:100
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IHC-P
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1:100-1:500
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FC
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 102 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Hexokinase II
SYNONYMS
DKFZp686M1669 antibody;Hexokinase 2 antibody;Hexokinase 2 muscle antibody;Hexokinase type II antibody;Hexokinase-2 antibody;HK 2 antibody;HK II antibody;HK2 antibody;HKII antibody;HxK 2 antibody;HxK2 antibody;HXK2_HUMAN antibody;Muscle form hexokinase antibody
SUBCELLULAR LOCATION
Cytosol, Mitochondrion outer membrane.
FUNCTION
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
CITATIONS
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Human
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Citation
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chicken
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Dan Wang,et al
Targeting SphK1/S1PR3 axis ameliorates sepsis-induced multiple organ injury via orchestration of macrophage polarization and glycolysis
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WB
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mice
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Citation
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