HER2 / ErbB2 Rabbit Polyclonal Antibody (ER0106)
Catalog# ER0106
Rabbit Polyclonal to HER2 / ErbB2
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WB
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IHC-P
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Human
Lane 1: SK-Br-3 cell lysate
Lane 2: SKOV-3 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 138 kDa
Observed band size: 180 kDa
Exposure time: 2 minutes;
6% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER0106) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
HER2 / ErbB2 Rabbit Polyclonal Antibody (ER0106)
Immunogen
Synthetic peptide within human her2 aa 1-50 / 1,255.
Host
Rabbit
Positive Control
SK-Br-3 cell lysate, SKOV-3 cell lysate, 4T1 cell lysate, human breast cancer tissue, human stomach carcinoma tissue, human placenta tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 138 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HER2 / ErbB2
SYNONYMS
Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody;C erb B2/neu protein antibody;CD340 antibody;CD340 antigen antibody;Cerb B2/neu protein antibody;CerbB2 antibody;Erb b2 receptor tyrosine kinase 2 antibody;ERBB2 antibody;ERBB2_HUMAN antibody;HER 2 antibody;HER 2/NEU antibody;HER2 antibody;Herstatin antibody;Human epidermal growth factor receptor 2 antibody;Metastatic lymph node gene 19 protein antibody;MLN 19 antibody;MLN19 antibody;NEU antibody;NEU proto oncogene antibody;Neuro/glioblastoma derived oncogene homolog antibody;Neuroblastoma/glioblastoma derived oncogene homolog antibody;NGL antibody;p185erbB2 antibody;Proto-oncogene c-ErbB-2 antibody;Proto-oncogene Neu antibody;Receptor tyrosine-protein kinase erbB-2 antibody;TKR1 antibody;Tyrosine kinase type cell surface receptor HER2 antibody;Tyrosine kinase-type cell surface receptor HER2 antibody;V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 (neuro/glioblastoma derived oncogene homolog) antibody;V erb b2 avian erythroblastic leukemia viral oncogene homolog 2 antibody;V erb b2 avian erythroblastic leukemia viral oncoprotein 2 antibody;V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody;V erb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog antibody;Verb b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.
TISSUE SPECIFICITY
Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.
POST-TRANSLATIONAL MODIFICATION
Autophosphorylated. Autophosphorylation occurs in trans, i.e. one subunit of the dimeric receptor phosphorylates tyrosine residues on the other subunit (Probable). Ligand-binding increases phosphorylation on tyrosine residues. Signaling via SEMA4C promotes phosphorylation at Tyr-1248. Dephosphorylated by PTPN12.
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
HER2 is a member of the human epidermal growth factor receptor (HER/EGFR/ERBB) family. Amplification or overexpression of this oncogene has been shown to play an important role in the development and progression of certain aggressive types of breast cancer. In recent years the protein has become an important biomarker and target of therapy for approx. 30% of breast cancer patients. Over-expression is also known to occur in ovarian, stomach, and aggressive forms of uterine cancer, such as uterine serous endometrial carcinoma. The expression of HER2 is regulated by signaling through estrogen receptors. Normally, estradiol and tamoxifen acting through the estrogen receptor down-regulate the expression of HER2. However, when the ratio of the coactivator AIB-3 exceeds that of the corepressor PAX2, the expression of HER2 is upregulated in the presence of tamoxifen, leading to tamoxifen-resistant breast cancer.
CITATIONS
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Wu, Y., Xue, J., Li, Y., Wu, X., Qu, M., Xu, D., & Shi, Y.
Expression, clinical significance and correlation of RUNX3 and HER2 in colorectal cancer
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IHC
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Human
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Citation
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Lin Wang,et al
Assembly of Dye Molecules in Covalent Organic Frameworks for Enhanced Colorimetric Biosensing
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Cheng Di,et al
Overexpression of miR-489-3p inhibits Proliferation and Migration of Non-Small Cell Lung Cancer cells by Suppressing the HER2/PI3K/AKT/Snail signaling Pathway
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WB
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Human
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Citation
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