Hemoglobin subunit alpha Recombinant Rabbit Monoclonal Antibody [PD00-16] (HA721183)
Catalog# HA721183
Rabbit Monoclonal to Hemoglobin subunit alpha
Tested for
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WB
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IHC-P
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of Hemoglobin subunit alpha on rat heart tissue lysates with Rabbit anti-Hemoglobin subunit alpha antibody (HA721183) at 1/2,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 12 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721183) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 15 kDa
Observed band size: 12 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721183) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.