Lane 1, 3: Anti-HEC1 antibody
Lane 2, 4: Anti-HEC1 antibody, with immunization Recombinant protein
Rabbit polyclonal primary
HEC1 Rabbit Polyclonal Antibody (ER1803-56)
Recombinant protein within human hec1 aa 300-505 / 642.
SiHa, HL-60, MG-63.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Protein affinity purified.
from Entrez Gene ; antibody; HEC antibody; Highly expressed in cancer antibody; Highly expressed in cancer protein antibody; Highly expressed in cancer rich in leucine heptad repeats antibody; HsHec1 antibody; hsNDC80 antibody; Kinetochore associated 2 antibody; Kinetochore associated protein 2 antibody; Kinetochore protein Hec1 antibody; Kinetochore protein NDC80 homolog antibody; Kinetochore-associated protein 2 antibody; KNTC2 antibody; ndc80 antibody; NDC80 homolog kinetochore complex component antibody; NDC80 kinetochore complex component homolog antibody; NDC80, S. cerevisiae, homolog of antibody; NDC80_HUMAN antibody; Retinoblastoma associated protein HEC antibody; Retinoblastoma-associated protein HEC antibody; TID3 antibody
Belongs to the NDC80/HEC1 family.
Expression peaks in mitosis.
Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.