PRODUCT CODE: ER1803-56

HEC1 Rabbit Polyclonal Antibody (ER1803-56)

Applications

  • WB

  • FC

REACTIVITY

  • Human

Western blot analysis of HEC1 on SiHa (1,2) and  HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1, 3: Anti-HEC1 antibody<br />
 Lane 2, 4: Anti-HEC1 antibody, with immunization Recombinant protein
  • Western blot analysis of HEC1 on SiHa (1,2) and  HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution.<br />
Positive control:<br />
 Lane 1, 3: Anti-HEC1 antibody<br />
 Lane 2, 4: Anti-HEC1 antibody, with immunization Recombinant protein
  • Flow cytometric analysis of MG-63 cells with HEC1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of HEC1 on SiHa (1,2) and HL-60 (3,4) cell lysates using anti-HEC1 antibody at 1/500 dilution.
Positive control:
Lane 1, 3: Anti-HEC1 antibody
Lane 2, 4: Anti-HEC1 antibody, with immunization Recombinant protein

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HEC1 Rabbit Polyclonal Antibody (ER1803-56)

Immunogen

Recombinant protein within human hec1 aa 300-505 / 642.

Host

Rabbit

Positive Control

SiHa, HL-60, MG-63.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

74 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

HEC1

SYNONYMS

from Entrez Gene ; antibody; HEC antibody; Highly expressed in cancer antibody; Highly expressed in cancer protein antibody; Highly expressed in cancer rich in leucine heptad repeats antibody; HsHec1 antibody; hsNDC80 antibody; Kinetochore associated 2 antibody; Kinetochore associated protein 2 antibody; Kinetochore protein Hec1 antibody; Kinetochore protein NDC80 homolog antibody; Kinetochore-associated protein 2 antibody; KNTC2 antibody; ndc80 antibody; NDC80 homolog kinetochore complex component antibody; NDC80 kinetochore complex component homolog antibody; NDC80, S. cerevisiae, homolog of antibody; NDC80_HUMAN antibody; Retinoblastoma associated protein HEC antibody; Retinoblastoma-associated protein HEC antibody; TID3 antibody

SEQUENCE SIMILARITIES

Belongs to the NDC80/HEC1 family.

DEVELOPMENTAL STAGE

Expression peaks in mitosis.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation begins in S phase of the cell cycle and peaks in mitosis. Phosphorylated by NEK2. May also be phosphorylated by AURKA and AURKB.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules. Plays a role in chromosome congression and is essential for the end-on attachment of the kinetochores to spindle microtubules.