Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HE4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1702-61, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Lane 1: SW480 cell lysate
Lane 2: SKOV-3 cell lysate
Lane 2: HepG2 cell lysate
dJ461P17.6 antibody;EDDM4 antibody;epididymal protein 4 antibody;Epididymal secretory protein E4 antibody;Epididymis specific whey acidic protein type four disulfide core antibody;HE 4 antibody;Major epididymis specific protein E4 antibody;Major epididymis-specific protein E4 antibody;MGC57529 antibody;Putative protease inhibitor WAP5 antibody;WAP 5 antibody;WAP domain containing protein HE4 antibody;WAP domain containing protein HE4 V4 antibody;WAP four disulfide core domain 2 antibody;WAP four disulfide core domain protein 2 antibody;WAP four-disulfide core domain protein 2 antibody;WAP5 antibody;WFDC 2 antibody;WFDC2 antibody;WFDC2_HUMAN antibody
Expressed in a number of normal tissues, including male reproductive system, regions of the respiratory tract and nasopharynx. Highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. Initially described as being exclusively transcribed in the epididymis.
HE4 (whey acidic protein (WAP)-type four-disulfide core-2, WFDC2) is a small secretory protein that may influence sperm maturation. HE4 gene expression is high in pulmonary epithelial cells and in some ovarian cancers. HE4 protein has a WAP motif that contains eight cysteines forming four disulfide bonds at the core of the protein. The WAP motif functions as a protease inhibitor in many of the family members that contain them.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.