HDAC3 Recombinant Rabbit Monoclonal Antibody [SC55-02] (ET1610-5)
Catalog# ET1610-5
Rabbit Monoclonal to HDAC3
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WB
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IF-Cell
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IF-Tissue
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HEK-293 cell lysate
Lane 4: HT-29 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C6 cell lysate
Lane 7: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1610-5) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HDAC3 Recombinant Rabbit Monoclonal Antibody [SC55-02] (ET1610-5)
Immunogen
Synthetic peptide within c-terminal human hdac3.
Host
Rabbit
Positive Control
HeLa cell lysate, K-562 cell lysate, HEK-293 cell lysate, HT-29 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, HeLa, human skin tissue, mouse skin tissue, rat skin tissue, human tonsil tissue, human breast carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SC55-02
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50
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IF-Tissue
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1:50-1:200
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IHC-P
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1:200-1:1,000
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IP
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Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 49 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HDAC3
SYNONYMS
HD3 antibody;HDAC 3 antibody;HDAC3 antibody;HDAC3_HUMAN antibody;Histone deacetylase 3 antibody;RPD3 2 antibody;RPD3 antibody;RPD3-2 antibody;SMAP45 antibody
SEQUENCE SIMILARITIES
Belongs to the histone deacetylase family. HD type 1 subfamily.
TISSUE SPECIFICITY
Widely expressed.
POST-TRANSLATIONAL MODIFICATION
Sumoylated in vitro.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus.
FUNCTION
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino-terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1), HDAC2 (also designated RPD3) and HDAC3, all of which are related to the yeast transcriptional factor Rpd3p, have been identified as histone deacetylases.
CITATIONS
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Professor Ping Fan
Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells
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WB
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Human
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Citation
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SERPINA3-ANKRD11-HDAC3 pathway induced aromatase inhibitor resistance in breast cancer can be reversed by HDAC3 inhibition
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WB
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Human
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Citation
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Vitamin A deficiency suppresses CEACAM1 to impair colonic epithelial barrier function via downregulating microbial-derived short-chain fatty acids
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WB
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Rat
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Citation
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Sodium butyrate facilitates CRHR2 expression to alleviate HPA axis hyperactivity in autism-like rats induced by prenatal lipopolysaccharides through histone deacetylase inhibition
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WB
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Rat
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Citation
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Liu, H., Tan, M., Cheng, B., Wang, S., Xiao, L., Zhu, J., Wu, Q., Lai, X., Zhang, Q., Chen, J., & Li, T.
Valproic Acid Induces Autism-Like Synaptic and Behavioral Deficits by Disrupting Histone Acetylation of Prefrontal Cortex ALDH1A1 in Rats. Frontiers in neuroscience, 15, 641284.
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WB
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Rat
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Citation
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Di Wang
Histone Deacetylase 3 Couples Mitochondria to Drive IL-1β-Dependent Inflammation by Configuring Fatty Acid Oxidation
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WB
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IP
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Mouse
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Citation
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Weikang Zou,et al
Role of HDAC3 in the epithelial-mesenchymal transition of retinal pigment epithelium cells: implications for proliferative vitreoretinopathy
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IP
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Mouse
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Citation
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Dr Ben-Jun Wang
Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells
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WB
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A549 and HCT116 cells
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Citation
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