PRODUCT CODE: ET1612-50

HDAC2 Recombinant Rabbit Monoclonal Antibody [SD0816] (ET1612-50)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: K562             <br />
Lane 2: Hela
  • Western blot analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution.<br />
Positive control:   <br />
Lane 1: K562             <br />
Lane 2: Hela
Western blot analysis of HDAC2 on different lysates using anti-HDAC2 antibody at 1/1,000 dilution.
Positive control:
Lane 1: K562
Lane 2: Hela

Applications

  • WB

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HDAC2 Recombinant Rabbit Monoclonal Antibody [SD0816] (ET1612-50)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

K562, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD0816

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

60 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

TARGET

UNIPROT #

PROTEIN NAME

HDAC2

SYNONYMS

D10Wsu179e antibody; HD 2 antibody; HD2 antibody; HDAC 2 antibody; Hdac2 antibody; HDAC2_HUMAN antibody; Histone deacetylase 2 (HD2) antibody; Histone deacetylase 2 antibody; OTTHUMP00000017046 antibody; OTTHUMP00000227077 antibody; OTTHUMP00000227078 antibody; RPD3 antibody; transcriptional regulator homolog RPD3 antibody; YAF1 antibody; YY1 associated factor 1 antibody; YY1 transcription factor binding protein antibody; Yy1bp antibody

SEQUENCE SIMILARITIES

Belongs to the histone deacetylase family. HD type 1 subfamily.

TISSUE SPECIFICITY

Widely expressed; lower levels in brain and lung.

POST-TRANSLATIONAL MODIFICATION

S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

SUBCELLULAR LOCATION

Nucleus, Cytoplasm.

FUNCTION

In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (for p300/CBP-associated factor), p300/CBP and the TFIID subunit TAF II p250. Mammalian HDAC1 (also designated HD1) and HDAC2 (also designated mammalian RPD3), both of which are related to the yeast transcriptional regulator Rpd3p, have been identified as histone deacetylases.