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PRODUCT CODE: ET1612-69

HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11] (ET1612-69)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of HDAC10 on HepG2 cells lysates using anti-HDAC10 antibody at 1/1,000 dilution.
  • Western blot analysis of HDAC10 on HepG2 cells lysates using anti-HDAC10 antibody at 1/1,000 dilution.
  • ICC staining HDAC10 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC10 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining HDAC10 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using anti-HDAC10 antibody. Counter stained with hematoxylin.
Western blot analysis of HDAC10 on HepG2 cells lysates using anti-HDAC10 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IP

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HDAC10 Recombinant Rabbit Monoclonal Antibody [SD08-11] (ET1612-69)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

A431, A549, Hela, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SD08-11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

43 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

Q969S8

PROTEIN NAME

HDAC10

SYNONYMS

DKFZP761B039 antibody; HD 10 antibody; HD10 antibody; HDA10_HUMAN antibody; HDAC 10 antibody; Hdac10 antibody; Histone deacetylase 10 antibody; MGC149722 antibody; OTTHUMP00000028555 antibody

SEQUENCE SIMILARITIES

Belongs to the histone deacetylase family. HD type 2 subfamily.

TISSUE SPECIFICITY

Widely expressed with high levels in liver and kidney.

SUBCELLULAR LOCATION

Cytoplasm, Nucleus.

FUNCTION

Histone deacetylases (HDACs) play an important role in the modification of chromatin structure and thus in the suppression and activation of transcription and cellular differentiation. There are 11 members in the HDAC family that are divided into four classes. Class I HDACs represent homologs of the yeast histone deacetylase Rpd3, class II HDACs share strong homology with the yeast histone deacetylase Hda1, class III HDACs are closely related to the yeast Sir2 protein and class IV HDACs comprise histone deacetylase 11 (HDAC11)-related enzymes. HDAC10, also known as HD10, is a member of the class II HDACs. It contains an N-terminal Hda1p-related catalytic domain and a unique C-terminal leucine-rich domain. HDAC10 is ubiquitously expressed and can shuttle between the cytoplasm and nucleus in response to celllular signals. It is able to repress transcription and, like other class II HDAC members, its enzymatic activity is inhibited by Trichostatin A (TSA).