HDAC1 Recombinant Rabbit Monoclonal Antibody [SY12-04] (ET1605-35)
Catalog# ET1605-35
Rabbit Monoclonal to HDAC1
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WB
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IHC-P
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IF-Cell
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IP
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IF-Tissue
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: L-929 cell lysate
Lane 6: C6 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 65 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1605-35) at 1/5,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% BSA at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
HDAC1 Recombinant Rabbit Monoclonal Antibody [SY12-04] (ET1605-35)
Immunogen
Synthetic peptide within human hdac1 aa 420-460.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, MCF7 cell lysate, Jurkat cell lysate, L929 cell lysate, C6 cell lysate, HeLa, NIH/3T3, C6, human colon tissue, human pancreas tissue, mouse colon tissue, mouse pancreas tissue, mouse lymph nodes tissue, rat liver tissue, rat pancreas tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY12-04
RRID
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:50-1:1,000
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IF-Cell
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1:100-1:500
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IP
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1-2μg/sample
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IF-Tissue
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 55 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
HDAC1
SYNONYMS
DKFZp686H12203 antibody;GON 10 antibody;HD1 antibody;HDAC 1 antibody;HDAC1 antibody;HDAC1_HUMAN antibody;Histone deacetylase 1 antibody;Reduced potassium dependency yeast homolog like 1 antibody;RPD3 antibody;RPD3L1 antibody
SEQUENCE SIMILARITIES
Belongs to the histone deacetylase family. HD type 1 subfamily.
TISSUE SPECIFICITY
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
POST-TRANSLATIONAL MODIFICATION
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.; Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Phosphorylated by CDK5.; Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by KCTD11, leading to proteasomal degradation.
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2 MTA2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.
CITATIONS
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Professor Ping Fan
Effect of sodium butyrate on ABC transporters in lung cancer A549 and colorectal cancer HCT116 cells
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WB
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Human
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Citation
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STAT3 phosphorylation mediates high glucose-impaired cell autophagy in an HDAC1-dependent and -independent manner in Schwann cells of diabetic peripheral neuropathy.
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WB
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ICC
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Rat
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Citation
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Jin Tingting,et al
HDAC1 Promotes Mitochondrial Pathway Apoptosis and Inhibits the Endoplasmic Reticulum Stress Response in High Glucose-Treated Schwann Cells via Decreased U4 Spliceosomal RNA
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IHC
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IF
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WB
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Mouse
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Citation
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Vitamin A deficiency suppresses CEACAM1 to impair colonic epithelial barrier function via downregulating microbial-derived short-chain fatty acids
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WB
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Rat
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Citation
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Sodium butyrate facilitates CRHR2 expression to alleviate HPA axis hyperactivity in autism-like rats induced by prenatal lipopolysaccharides through histone deacetylase inhibition
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WB
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Rat
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Citation
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Liu, H., Tan, M., Cheng, B., Wang, S., Xiao, L., Zhu, J., Wu, Q., Lai, X., Zhang, Q., Chen, J., & Li, T.
Valproic Acid Induces Autism-Like Synaptic and Behavioral Deficits by Disrupting Histone Acetylation of Prefrontal Cortex ALDH1A1 in Rats. Frontiers in neuroscience, 15, 641284.
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WB
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Rat
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Citation
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Dr Ben-Jun Wang
Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells
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WB
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A549 and HCT116 cells
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Citation
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