Lane 2: MCF-7 cell lysate
Lane 2: Hela cell lysate
Lane 2: Jurkat cell lysate
Lane 2: K562 cell lysate
Recombinant Rabbit monoclonal primary
HDAC1 Recombinant Rabbit Monoclonal Antibody [SY12-04] (ET1605-35)
MCF-7 cell lysate, Hela cell lysate, Jurkat cell lysate, K562 cell lysate, human colon tissue, mouse colon tissue, mouse pancreas tissue, human pancreas tissue.
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Protein A affinity purified.
DKFZp686H12203 antibody; GON 10 antibody; HD1 antibody; HDAC 1 antibody; HDAC1 antibody; HDAC1_HUMAN antibody; Histone deacetylase 1 antibody; Reduced potassium dependency yeast homolog like 1 antibody; RPD3 antibody; RPD3L1 antibody
Belongs to the histone deacetylase family. HD type 1 subfamily.
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.; Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes. Phosphorylated by CDK5.; Ubiquitinated by CHFR, leading to its degradation by the proteasome. Ubiquitinated by KCTD11, leading to proteasomal degradation.
Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to DNA. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription. HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes. In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity. Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases. Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents.
Wang, Hao et al.
Effects of histone deacetylase inhibitors on ATP-binding cassette transporters in lung cancer A549 and colorectal cancer HCT116 cells. | Oncology Letters