Positive control:
Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Applications
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WB
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IHC-P
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ICC/IF
REACTIVITY
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Human
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Mouse
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Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Haptoglobin Recombinant Rabbit Monoclonal Antibody [JM10-79] (ET1703-24)
Immunogen
Synthetic peptide within human haptoglobin aa 357-406 / 406.
Host
Rabbit
Positive Control
Hela cell lysate, HepG2 cell lysate, A549, Hela, HepG2, human liver tissue, human lung tissue, mouse liver tissue, mouse lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM10-79
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A affinity purified.
MOLECULAR WEIGHT
45/38 kDa
Isotype
IgG
APPLICATION DILUTION
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WB
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1:500-1:2,000
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ICC/IF
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1:50-1:200
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IHC-P
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1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
Haptoglobin
SYNONYMS
Binding peptide antibody; BP antibody; Haptoglobin alpha chain antibody; Haptoglobin alpha(1S) beta antibody; Haptoglobin alpha(2FS) beta antibody; Haptoglobin beta chain antibody; Haptoglobin, alpha polypeptide antibody; Haptoglobin, beta polypeptide antibody; HP antibody; HP2 ALPHA2 antibody; HP2ALPHA2 antibody; HPA1S antibody; HPT antibody; HPT_HUMAN antibody; MGC111141 antibody; Zonulin antibody
SEQUENCE SIMILARITIES
Belongs to the peptidase S1 family.
TISSUE SPECIFICITY
Expressed by the liver and secreted in plasma.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Haptoglobin (Hp) is a blood plasma protein that functions to bind free Hemoglobin that has been released from erythrocytes, thereby inhibiting its oxidative activity. During this process, Haptoglobin sequesters the iron within Hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. This function suggests that Haptoglobin concentrations may increase in response to inflammation. The resulting Haptoglobin-Hemoglobin complex is then removed by the reticulo-endothelial system. Due to cleavage of a common precursor protein during protein synthesis, Haptoglobin consists of two α and two β chains, connected by disulfide bridges. In human, Haptoglobin exists in two allelic forms designated Haptoglogin 1 (Hp1) and Haptoglobin 2 (Hp2), where Hp2 is the result of a partial Hp1 gene duplication. There are three known phenotypes of human Haptoglobin: Hp1-1, Hp2-1 and Hp2-2, which may be associated with diabetes and cardiovascular disease pathology and a susceptibility to Parkinson's and Crohn's disease. Haptoglobin levels are useful in diagnosing hemolytic anemia, the abnormal breakdown of red blood cells. Haptoglobin is expressed in mammalian hepatocytes as well as other tissues such as skin, lung and kidney.
CITATIONS
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Zheng, Xi et al.
A circulating extracellular vesicles-based novel screening tool for colorectal cancer revealed by shotgun and data-independent acquisition mass spectrometry. | Journal of Extracellular Vesicles [2020]
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WB
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human
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Citation
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