PRODUCT CODE: ET1607-7

HAPLN1 Recombinant Rabbit Monoclonal Antibody [SY02-17] (ET1607-7)

  • Zebrafish
  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

Western blot analysis of HAPLN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: BT-20 cell lysate<br />
Lane 2: HUVEC cell lysate<br />
Lane 3: SW480 cell lysate
  • Western blot analysis of HAPLN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: BT-20 cell lysate<br />
Lane 2: HUVEC cell lysate<br />
Lane 3: SW480 cell lysate
  • ICC staining of HAPLN1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-7, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of HAPLN1 in SW480 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-7, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of HAPLN1 in BT-20 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1607-7, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-7, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of HAPLN1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1607-7, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: BT-20 cell lysate
Lane 2: HUVEC cell lysate
Lane 3: SW480 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

  • Zebrafish

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HAPLN1 Recombinant Rabbit Monoclonal Antibody [SY02-17] (ET1607-7)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

BT-20 cell lysate, HUVEC cell lysate, SW480 cell lysate, HUVEC, SW480, BT-20, human tonsil tissue, human liver tissue, human spleen tissue, mouse spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SY02-17

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

48 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

TARGET

UNIPROT #

PROTEIN NAME

HAPLN1

SYNONYMS

Cartilage link protein antibody; cartilage linking protein 1 antibody; Cartilage-link protein antibody; Cartilage-linking protein 1 antibody; CLP antibody; CRTL1 antibody; Crtl1l antibody; HAPLN1 antibody; HPLN1_HUMAN antibody; Hyaluronan and proteoglycan link protein 1 antibody; Hyaluronan and proteoglycan link protein 1 precursor antibody; LP antibody; Proteoglycan link protein antibody

SEQUENCE SIMILARITIES

Belongs to the HAPLN family.

TISSUE SPECIFICITY

Widely expressed. Weakly expressed in the brain.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

The human cartilage link protein, also designated HAPLN1, is a member of the hyaluronan and proteoglycan link protein (HAPLN) family of proteins. This family contains 4 proteins with approximately 50% homology. The human cartilage link protein strengthens tissue architecture by using hyaluronic acid to stabilize the aggregates of proteoglycan monomers inside the extracellular cartilage matrix. It is widely expressed in most tissue types except brain tissue where, much like the other human cartilage link protein genes, it is weakly expressed. The HAPLN and the brain-specific CSPG genes, as well as other members of the link module superfamily, appear to have a common ancestral gene origin.