PRODUCT CODE: ET1703-12

HRP Conjugated HA-Tag Recombinant Rabbit Monoclonal Antibody [JM10-56] (ET1703-12)

Recombinant

Applications

WB

REACTIVITY

Western blot analysis of HA-tag on HA-tag recombinant protein lysates using anti-HA-tag antibody at 1/10,000 dilution.

Western blot analysis of HA-tag on HA-tag recombinant protein lysates using anti-HA-tag antibody at 1/10,000 dilution.

Applications

WB

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HRP Conjugated HA-Tag Recombinant Rabbit Monoclonal Antibody [JM10-56] (ET1703-12)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

HA-tag recombinant protein.

Conjugation

Hrp

Clonality

Monoclonal

Clone Number

JM10-56

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol.

Concentration

1ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

WB
1:5,000-1:10,000

TARGET

PROTEIN NAME

HA-Tag(HRP conjugated)

SYNONYMS

HA epitope tag antibody;HA1 antibody;HA2 antibody;hemagglutinin antibody;Hemagglutinin HA1 chain antibody;Hemagglutinin HA2 chain antibody

FUNCTION

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.

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ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754] (ET1611-58)

Applications

WB
ICC
IHC-P
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REACTIVITY

Human
Mouse
Hamster
Rat

Western blot analysis of ACE2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: human kidney tissue lysate<br />
Lane 2: human small intestine tissue lysate

NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23] (ET1610-93)

Applications

WB
ICC/IF
IHC-P
IP
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

CD9 Recombinant Rabbit Monoclonal Antibody [SA35-08] (ET1601-9)

Applications

WB
ICC/IF
IHC-P
IP
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of CD9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse heart tissue lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: human lung carcinoma tissue lysate<br />
Lane 4: human lung tissue lysate<br />
Lane 5: MCF-7 cell lysate

Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377] (ET1601-10)

Applications

WB
ICC/IF
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of Glucose Transporter GLUT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Sk-Br-3 cell lysate<br />
Lane 3: NIH/3T3 cell lysate<br />
Lane 4: HepG2 cell lysate

TAPA1/CD81 Recombinant Rabbit Monoclonal Antibody [SN206-01] (ET1611-87)

Applications

WB
ICC
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of TAPA1/CD81 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: PC-12 cell lysate<br />
Lane 2: JAR cell lysate

TLR2 Recombinant Rabbit Monoclonal Antibody [JM22-41] (ET1705-92)

Applications

WB
ICC/IF
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

All lanes: Western blot analysis of TLR2 with anti-TLR2 antibody [JM22-41] (ET1705-92) at 1:500 dilution.<br />
Lane 1: Wild-type THP-1 whole cell lysate.<br />
Lane 2: TLR2 knockout THP-1 whole cell lysate.<br />
<br />
ET1705-92 was shown to specifically react with TLR2 in wild-type THP-1 cells. No band was observed when TLR2 knockout samples were tested. Wild-type and TLR2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-TLR2 antibody (ET1705-92, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

PRODUCT CODE: ET1703-12

HRP Conjugated HA-Tag Recombinant Rabbit Monoclonal Antibody [JM10-56] (ET1703-12)

Recombinant

$379

100ul

Qty

Bulk Order Quote | Customization Request

All products are testedand guaranteed

RELATED PRODUCTS

ACE2 Recombinant Rabbit Monoclonal Antibody [SN0754] (ET1611-58)

Applications

WB
ICC
IHC-P
IP

REACTIVITY

Human
Mouse
Hamster
Rat

Western blot analysis of ACE2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-58, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: human kidney tissue lysate<br />
Lane 2: human small intestine tissue lysate

NLRP3 Recombinant Rabbit Monoclonal Antibody [SC06-23] (ET1610-93)

Applications

WB
ICC/IF
IHC-P
IP
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of NLRP3 on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1610-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

CD9 Recombinant Rabbit Monoclonal Antibody [SA35-08] (ET1601-9)

Applications

WB
ICC/IF
IHC-P
IP
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of CD9 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-9, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: mouse heart tissue lysate<br />
Lane 2: Jurkat cell lysate<br />
Lane 3: human lung carcinoma tissue lysate<br />
Lane 4: human lung tissue lysate<br />
Lane 5: MCF-7 cell lysate

Glucose Transporter GLUT1 Recombinant Rabbit Monoclonal Antibody [SA0377] (ET1601-10)

Applications

WB
ICC/IF
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of Glucose Transporter GLUT1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1601-10, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: Sk-Br-3 cell lysate<br />
Lane 3: NIH/3T3 cell lysate<br />
Lane 4: HepG2 cell lysate

TAPA1/CD81 Recombinant Rabbit Monoclonal Antibody [SN206-01] (ET1611-87)

Applications

WB
ICC
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

Western blot analysis of TAPA1/CD81 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-87, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: PC-12 cell lysate<br />
Lane 2: JAR cell lysate

TLR2 Recombinant Rabbit Monoclonal Antibody [JM22-41] (ET1705-92)

Applications

WB
ICC/IF
IHC-P
FC

REACTIVITY

Human
Mouse
Rat

All lanes: Western blot analysis of TLR2 with anti-TLR2 antibody [JM22-41] (ET1705-92) at 1:500 dilution.<br />
Lane 1: Wild-type THP-1 whole cell lysate.<br />
Lane 2: TLR2 knockout THP-1 whole cell lysate.<br />
<br />
ET1705-92 was shown to specifically react with TLR2 in wild-type THP-1 cells. No band was observed when TLR2 knockout samples were tested. Wild-type and TLR2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-TLR2 antibody (ET1705-92, 1/500) and Anti-β-actin antibody (R1207-1, 1/1,000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.