PRODUCT CODE: 0906-1

HA Tag Rabbit Polyclonal Antibody (0906-1)

Applications

  • WB

  • IP

REACTIVITY

Western blot analysis of HA Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: C-terminal HA-tagged recombinant protein<br />
Lane 2: N-terminal HA-tagged recombinant protein
  • Western blot analysis of HA Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: C-terminal HA-tagged recombinant protein<br />
Lane 2: N-terminal HA-tagged recombinant protein
  • HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with 0906-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.<br />
<br />
Lane 1: HA Tag fusion protein lysate (input).<br />
Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.<br />
Lane 3: 0906-1 IP in HA Tag fusion protein lysate.<br />
Lane 4: Rabbit IgG instead of 0906-1 in HA Tag fusion protein lysate.<br />
<br />
Blocking/Dilution buffer: 5% NFDM/TBST
Western blot analysis of HA Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal HA-tagged recombinant protein
Lane 2: N-terminal HA-tagged recombinant protein

Applications

  • WB

  • IP

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HA Tag Rabbit Polyclonal Antibody (0906-1)

Immunogen

Synthetic peptide immune sequence is ypydvpdyac.

Host

Rabbit

Positive Control

C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:10,000

  • IP

  • 2-5 µg/ml.

TARGET

PROTEIN NAME

HA Tag

FUNCTION

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.

CITATIONS

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    Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells. | Journal of Proteomics [2016]

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    Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis. Cellular oncology (Dordrecht), 42(4), 537–554.

    • WB

    • CO-IP

    • IHC

    • IF

    • Human hepatocellular carcinoma cell

    • Citation

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    Stk24 protects against obesity-associated metabolic disorders by disrupting the NLRP3 inflammasome. Cell reports, 35(8), 109161.

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    Pharmacological targeting of MCL-1 promotes mitophagy and improves disease pathologies in an Alzheimer’s disease mouse model

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    Interaction of AIM with insulin-like growth factor-binding protein-4. | International Journal of Molecular Medicine [2015]

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    Edwardsiella piscicida Type III Secretion System Effector EseK Inhibits Mitogen-Activated Protein Kinase Phosphorylation and Promotes Bacterial Colonization in Zebrafish Larvae. | Infection and Immunity [2018]

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    Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome. | Immunity [2016]

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    Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]