Positive control:
Lane 1: C-terminal HA-tagged recombinant protein
Lane 2: N-terminal HA-tagged recombinant protein
Applications
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WB
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IP
SPECIFICATIONS
Product Type
Rabbit polyclonal primary
Product Name
HA Tag Rabbit Polyclonal Antibody (0906-1)
Immunogen
Synthetic peptide immune sequence is ypydvpdyac.
Host
Rabbit
Positive Control
C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein.
Conjugation
Unconjugated
Clonality
Polyclonal
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Immunogen affinity purified
Isotype
IgG
APPLICATION DILUTION
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WB
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1:2,000-1:10,000
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IP
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2-5 µg/ml.
TARGET
PROTEIN NAME
HA Tag
FUNCTION
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.
CITATIONS
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Jian-Li Jiang,Hong-Yong Cu,...
Hypo-phosphorylated CD147 promotes migration and invasion of hepatocellular carcinoma cells and predicts a poor prognosis. Cellular oncology (Dordrecht), 42(4), 537–554.
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WB
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CO-IP
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IHC
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IF
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Human hepatocellular carcinoma cell
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Citation
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-
Zhou, Yanqing et al.
Quantitative proteomics identifies myoferlin as a novel regulator of A Disintegrin and Metalloproteinase 12 in HeLa cells. | Journal of Proteomics [2016]
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WB
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IP
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human
-
Citation
-
-
Guo, Chuansheng et al.
Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages. | Immunity [2018]
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WB
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None
-
Citation
-
-
Yang, Dahai et al.
The Edwardsiella piscicida thioredoxin-like protein inhibits ASK1-MAPKs signaling cascades to promote pathogenesis during infection. | PloS Pathogens [2019]
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WB
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HEK293T
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Citation
-
-
Wu, Y., Zhang, C., Liu, X.,...
ARIH1 signaling promotes anti-tumor immunity by targeting PD-L1 for proteasomal degradation. Nature communications, 12(1), 2346.
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WB
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Human
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Citation
-
-
Yang, S., Yan, H., Wu, Y., ...
Deubiquitination and Stabilization of PD-L1 by USP21
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WB
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Human
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Citation
-
-
You, Zhiyuan et al.
Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress. | Nature Communications [2019]
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IP
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CO-IP
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human
-
Citation
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-
Zhou, D., Yan, H., Yang, S....
SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway
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Cao, Huifang et al.
Edwardsiella piscicida Type III Secretion System Effector EseK Inhibits Mitogen-Activated Protein Kinase Phosphorylation and Promotes Bacterial Colonization in Zebrafish Larvae. | Infection and Immunity [2018]
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Zhou, D., Yan, H., Yang, S....
SC75741, A Novel c-Abl Inhibitor, Promotes the Clearance of TDP25 Aggregates via ATG5-Dependent Autophagy Pathway
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WB
-
Human
-
Citation
-
-
Hou, Mingyu et al.
Phosphothreonine Lyase Promotes p65 Degradation in a Mitogen-Activated Protein Kinase/Mitogen- and Stress-Activated Protein Kinase 1-Dependent Manner. | Infection and Immunity [2019]
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WB
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Escherichia coli
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Citation
-
-
Jie Wu ;Ayaz Najafov;Honggu...
Pharmacological targeting of MCL-1 promotes mitophagy and improves disease pathologies in an Alzheimer’s disease mouse model
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WB
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COIP
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human
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Citation
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-
Qin, Q., Shou, J., Li, M., ...
Stk24 protects against obesity-associated metabolic disorders by disrupting the NLRP3 inflammasome. Cell reports, 35(8), 109161.
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WB
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Citation
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-
Guo, Chuansheng et al.
Bile Acids Control Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome. | Immunity [2016]
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WB
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human
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Citation
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You, Qiang et al.
Interaction of AIM with insulin-like growth factor-binding protein-4. | International Journal of Molecular Medicine [2015]
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Co-IP
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WB
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human
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Citation
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-
Fu-Liang Hu
Polyphenol-rich propolis extracts from China and Brazil exert anti-inflammatory effects by modulating ubiquitination of TRAF6 during the activation of NF-κB
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IHC
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ICR mice
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Citation
-
-
You, Zhiyuan et al.
TP53INP2 contributes to autophagosome formation by promoting LC3-ATG7 interaction. | Autophagy [2019]
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WB
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HEK293 cells
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Citation
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