PRODUCT CODE: 0906-1

HA Tag Rabbit Polyclonal Antibody (0906-1)

Applications

  • WB

REACTIVITY

Western blot analysis of HA Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: C-terminal HA-tagged recombinant protein<br />
Lane 2: N-terminal HA-tagged recombinant protein
  • Western blot analysis of HA Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control:<br />
Lane 1: C-terminal HA-tagged recombinant protein<br />
Lane 2: N-terminal HA-tagged recombinant protein
Western blot analysis of HA Tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (0906-1, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: C-terminal HA-tagged recombinant protein
Lane 2: N-terminal HA-tagged recombinant protein

Applications

  • WB

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

HA Tag Rabbit Polyclonal Antibody (0906-1)

Immunogen

Synthetic peptide immune sequence is ypydvpdyac.

Host

Rabbit

Positive Control

C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-1:10,000

TARGET

PROTEIN NAME

HA Tag

FUNCTION

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.

CITATIONS

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  • Cao, Huifang et al.

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    Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress. | Nature Communications [2019]