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PRODUCT CODE: ET1611-49

HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06] (ET1611-49)

  • Recombinant

Applications

  • WB

  • IP

REACTIVITY

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Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of HA tag on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: C-terminal HA-tagged recombinant protein<br /> Lane 2: N-terminal HA-tagged recombinant protein
  • HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with ET1611-49 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using M1008-1 at 1/1000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:20,000 dilution was used for 60 mins at room temperature.<br /> <br /> Lane 1: HA Tag fusion protein lysate (input).<br /> Lane 2: ET1611-49 IP in HA Tag fusion protein lysate.<br /> Lane 3: 0906-1 IP in HA Tag fusion protein lysate.<br /> Lane 4: Rabbit IgG instead of ET1611-49 in HA Tag fusion protein lysate.<br /> <br /> Blocking/Dilution buffer: 5% NFDM/TBST
Western blot analysis of HA tag on HA tag recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-49) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IP

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

HA tag Recombinant Rabbit Monoclonal Antibody [SN07-06] (ET1611-49)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

HA tag recombinant protein, C-terminal HA-tagged recombinant protein, N-terminal HA-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN07-06

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • IP

  • 2-5 µg/ml.

TARGET

PROTEIN NAME

HA tag

SYNONYMS

HA epitope tag antibody; HA1 antibody; HA2 antibody; hemagglutinin antibody; Hemagglutinin HA1 chain antibody; Hemagglutinin HA2 chain antibody

FUNCTION

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA tag recombinant protein.

CITATIONS

  • Fu, Guotong et al.

    Suppression of Th17 cell differentiation by misshapen/NIK-related kinase MINK1. | The Journal of Experimental Medicine [2017]

  • Mingliang Zhang;Dan Ye

    The Zscan4-Tet2 Transcription Nexus RegulatesMetabolic Rewiring and Enhances Proteostasis toPromote Reprogramming