HA tag Recombinant Rabbit Multiclonal Antibody [PSH01-92] (HA721750)
Catalog# HA721750
Rabbit to HA tag
Tested for
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WB
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IF-Cell
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IP
Reacts with
-
Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of HA tag on different lysates with Rabbit anti-HA tag antibody (HA721750) at 1/5,000 dilution.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
Lane 4: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate
Lane 5: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721750) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate
Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
Lane 4: 293T transfected with HA-tagged CXCL13 (C-terminal) cell lysate
Lane 5: 293T transfected with HA-tagged CLK4 (C-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721750) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.