PRODUCT CODE: M1008-1

HA Tag Mouse Monoclonal Antibody [2-G10] (M1008-1)

Applications

  • WB

  • IP

  • ICC

REACTIVITY

Western blot analysis on recombinant protein with HA tag using anti-HA tag Mouse mAb (Cat. # M1008-1).
  • Western blot analysis on recombinant protein with HA tag using anti-HA tag Mouse mAb (Cat. # M1008-1).
  • HA tag was immunoprecipitated in 5µg C terminal HA Tag fusion protein lysate with M1008-1 at 2 µg/20 µl agarose. Western blot was performed from the immunoprecipitate using ET1611-49 at 1/500 dilution. Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 60 mins at room temperature.<br />
<br />
Lane 1: HA Tag fusion protein lysate (input).<br />
Lane 2: M1008-1 IP in HA Tag fusion protein lysate.<br />
Lane 3: Mouse IgG instead of M1008-1 in HA Tag fusion protein lysate.<br />
<br />
Blocking/Dilution buffer: 5% NFDM/TBST
  • Immunocytochemistry analysis of HA-Gag transfected Hela cells labeling HA Tag with Mouse anti-HA Tag antibody (M1008-1) at 1/100 dilution.<br />
<br />
Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-HA Tag antibody (M1008-1) at 1/100 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Western blot analysis on recombinant protein with HA tag using anti-HA tag Mouse mAb (Cat. # M1008-1).

Applications

  • WB

  • IP

  • ICC

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

HA Tag Mouse Monoclonal Antibody [2-G10] (M1008-1)

Immunogen

Synthetic peptide corresponding to ha tag conjugated to klh.

Host

Mouse

Positive Control

Recombinant protein with HA tag, HA-Gag transfected Hela cells.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

2-G10

PROPERTIES

Form

Liquid

Storage Condition

Store at -20 C. Stable for 12 months from date of receipt.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2ug/ul

PURIFICATION

Protein G affinity purified.

Isotype

IgM

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IP

  • 2-5 µg/ml.

  • ICC

  • 1:100

TARGET

PROTEIN NAME

HA Tag

SYNONYMS

HA epitope tag antibody;HA1 antibody;HA2 antibody;hemagglutinin antibody;Hemagglutinin HA1 chain antibody;Hemagglutinin HA2 chain antibody

FUNCTION

Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.

CITATIONS

  • Yu, D et al.

    Dual roles of misshapen/NIK-related kinase (MINK1) in osteoarthritis subtypes through the activation of TGF-β_ signaling. | Osteoarthritis and Cartilage [2020]