HA tag Recombinant Mouse Monoclonal Antibody [2-G10] (M1008-1)
Catalog# M1008-1
Mouse Monoclonal to HA tag
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WB
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IHC-P
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IF-Cell
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Species independent
Lane 1: 293T cell lysate
Lane 2: 293T transfected with HA-tagged Nanos homolog 3 (N-terminal) cell lysate
Lane 3: 293T transfected with HA-tagged LIPT1 (N-terminal) cell lysate
Lysates/proteins at 10 µg/Lane.
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1008-1) at 1/10,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
HA tag Recombinant Mouse Monoclonal Antibody [2-G10] (M1008-1)
Immunogen
Synthetic peptide corresponding to ha tag conjugated to klh.
Host
Mouse
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
2-G10
RRID
APPLICATION DILUTION
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WB
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1:10,000-1:25,000
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IHC-P
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1:1,000-1:10,000
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IF-Cell
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein G affinity purified.
Isotype
IgG1
TARGET
PROTEIN NAME
HA tag
SYNONYMS
HA epitope tag antibody;HA1 antibody;HA2 antibody;hemagglutinin antibody;Hemagglutinin HA1 chain antibody;Hemagglutinin HA2 chain antibody
FUNCTION
Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106. This antibody is used to detect proteins that are tagged with HA epitope (YPYDVPDYA) and are expressed in prokaryotic and eukaryotic cells.
CITATIONS
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Prof. Hong Wei Ouyang ;Prof. Lin Rong Lu
Dual roles of misshapen/NIK-related kinase (MINK1) in osteoarthritis subtypes through the activation of TGFβ signaling.
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WB
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Mouse
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Citation
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