GTPase HRAS Recombinant Rabbit Monoclonal Antibody [JE42-15] (HA721355)

UNIPROT #

P01112

PROTEIN NAME

GTPase HRAS

SYNONYMS

C BAS/HAS antibody;c H ras antibody;C HA RAS1 antibody;c has/bas p21 protein antibody;c ras Ki 2 activated oncogene antibody;c-H-ras antibody;CTLO antibody;GTP and GDP binding peptide B antibody;GTPase HRas, N-terminally processed antibody;H Ras 1 antibody;H RASIDX antibody;H-Ras-1 antibody;Ha Ras antibody;Ha Ras1 proto oncoprotein antibody;Ha-Ras antibody;HAMSV antibody;Harvey rat sarcoma viral oncogene homolog antibody;Harvey rat sarcoma viral oncoprotein antibody;HRAS antibody;HRAS1 antibody;K ras antibody;N ras antibody;p19 H RasIDX protein antibody;p21ras antibody;Ras family small GTP binding protein H Ras antibody;RASH_HUMAN antibody;RASH1 antibody;Transformation gene oncogene HAMSV antibody;Transforming protein p21 antibody;v Ha ras Harvey rat sarcoma viral oncogene homolog antibody;VH Ras antibody;vHa RAS antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Ras family.

POST-TRANSLATIONAL MODIFICATION

Palmitoylated by the ZDHHC9-GOLGA7 complex. Depalmitoylated by ABHD17A, ABHD17B and ABHD17C. A continuous cycle of de- and re-palmitoylation regulates rapid exchange between plasma membrane and Golgi.; Acetylation at Lys-104 prevents interaction with guanine nucleotide exchange factors (GEFs).; Ubiquitinated by the BCR(LZTR1) E3 ubiquitin ligase complex at Lys-170 in a non-degradative manner, leading to inhibit Ras signaling by decreasing Ras association with membranes.; Phosphorylation at Ser-89 by STK19 enhances NRAS-association with its downstream effectors.

SUBCELLULAR LOCATION

Cell membrane, Golgi apparatus, Golgi apparatus membrane; Nucleus, Cytoplasm, perinuclear region.

FUNCTION

GTPase HRas, from "Harvey Rat sarcoma virus", also known as transforming protein p21 is an enzyme that in humans is encoded by the HRAS gene. The HRAS gene is located on the short (p) arm of chromosome 11 at position 15.5, from base pair 522,241 to base pair 525,549. HRas is a small G protein in the Ras subfamily of the Ras superfamily of small GTPases. Once bound to Guanosine triphosphate, H-Ras will activate a Raf kinase like c-Raf, the next step in the MAPK/ERK pathway. GTPase HRas is involved in regulating cell division in response to growth factor stimulation. Growth factors act by binding cell surface receptors that span the cell's plasma membrane. Once activated, receptors stimulate signal transduction events in the cytoplasm, a process by which proteins and second messengers relay signals from outside the cell to the cell nucleus and instructs the cell to grow or divide. The HRAS protein is a GTPase and is an early player in many signal transduction pathways and is usually associated with cell membranes due to the presence of an isoprenyl group on its C-terminus. HRAS acts as a molecular on/off switch, once it is turned on it recruits and activates proteins necessary for the propagation of the receptor's signal, such as c-Raf and PI 3-kinase. HRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity that cleaves the terminal phosphate of this nucleotide converting it to GDP. Upon conversion of GTP to GDP, HRAS is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein (GAP) class, for example RasGAP. In turn HRAS can bind to proteins of the Guanine Nucleotide Exchange Factor (GEF) class, for example SOS1, which forces the release of bound nucleotide. Subsequently, GTP present in the cytosol binds and HRAS-GTP dissociates from the GEF, resulting in HRAS activation. HRAS is in the Ras family, which also includes two other proto-oncogenes: KRAS and NRAS. These proteins all are regulated in the same manner and appear to differ largely in their sites of action within the cell.