GST3 Recombinant Rabbit Monoclonal Antibody [JB40-79] (ET7107-71)
Catalog# ET7107-71
Rabbit Monoclonal to GST3
Tested for
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WB
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IHC-P
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IF-Cell
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IF-Tissue
Reacts with
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Human
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Mouse
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Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GST3 on different lysates with Rabbit anti-GST3 antibody (ET7107-71) at 1/1,000 dilution.
Lane 1: HCT 116 cell lysate
Lane 1: HT-29 cell lysate
Lane 1: K-562 cell lysate
Lane 1: A549 cell lysate
Lane 2: Mouse liver tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane1-4 and 40ug/Lane5-7.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HCT 116 cell lysate
Lane 1: HT-29 cell lysate
Lane 1: K-562 cell lysate
Lane 1: A549 cell lysate
Lane 2: Mouse liver tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane1-4 and 40ug/Lane5-7.
Predicted band size: 23 kDa
Observed band size: 25 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7107-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.