GST Rabbit Polyclonal Antibody (1003-8)
Catalog# 1003-8
Rabbit Polyclonal to GST
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WB
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ELISA
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IP
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Species independent
Lane 1: 293T transfected with GST-tagged Histone H3.1 cell lysate (input)
Lane 2: Rabbit IgG instead of 1003-8 in 293T transfected with GST-tagged Histone H3.1 cell lysate
Lane 3: 1003-8 IP in 293T transfected with GST-tagged Histone H3.1 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 24 seconds
Specifications
Product Type
Rabbit polyclonal primary
Product Name
GST Rabbit Polyclonal Antibody (1003-8)
Immunogen
Recombinant protein within gst protein.
Host
Rabbit
Positive Control
GST fusion-protein
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:10,000-1:100,000
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ELISA
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1:10,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 25% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Isotype
IgG
TARGET
PROTEIN NAME
GST
SYNONYMS
Glutathione S Transferase antibody;Glutathione S-transferase class-mu 26 kDa isozyme antibody;GST antibody;Sj26 antigen antibody;SjGST antibody
FUNCTION
The pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase (GST) and proteins encoded by inserted cDNA sequences. Anti-bodies derived from these GST fusion proteins are useful for checking protein expression both in plaques and on Western blots, as well as for immuno affinity purification of proteins expressed in E. coli.
CITATIONS
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Ying Sun
The Receptor-Like Kinase SIT1 Mediates Salt Sensitivity by Activating MAPK3/6 and Regulating Ethylene Homeostasis in Rice
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WB
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rice
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Citation
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Wu Jiaqi，et al
The MoLfa1 Protein Regulates Fungal Development and Septin Ring Formation in Magnaporthe oryzae
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WB
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Citation
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Lu, Z. Z., Sun, C., Zhang, X., Peng, Y., Wang, Y., Zeng, Y., Zhu, N., Yuan, Y., & Zeng, M. S.
Neuropilin 1 is an entry receptor for KSHV infection of mesenchymal stem cell through TGFBR1/2-mediated macropinocytosis
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Chen Shue,et al
Dynamic interaction of REEP5-MFN1/2 enables mitochondrial hitchhiking on tubular ER
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WB
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Species independent
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Citation
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