PRODUCT CODE: ET1611-47

GST Recombinant Rabbit Monoclonal Antibody [SN70-05] (ET1611-47)

  • Recombinant

Applications

  • WB

  • ICC

  • IP

REACTIVITY

Western blot analysis of GST on GST tag recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-47, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of GST on GST tag recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-47, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GST on GST tag recombinant protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-47, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IP

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GST Recombinant Rabbit Monoclonal Antibody [SN70-05] (ET1611-47)

Immunogen

Recombinant protein.

Host

Rabbit

Positive Control

GST tag recombinant protein

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN70-05

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • ICC

  • 1:50-1:200

TARGET

PROTEIN NAME

GST

SYNONYMS

Glutathione S Transferase antibody; Glutathione S-transferase class-mu 26 kDa isozyme antibody; GST antibody; Sj26 antigen antibody; SjGST antibody

FUNCTION

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.