PRODUCT CODE: EM80701

GST Mouse Monoclonal Antibody [16-21] (EM80701)

Applications

  • WB

  • ELISA

REACTIVITY

Western blot analysis of GST on GST-tagged recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM80701, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of GST on GST-tagged recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM80701, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
Western blot analysis of GST on GST-tagged recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (EM80701, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ELISA

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GST Mouse Monoclonal Antibody [16-21] (EM80701)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

GST-tagged recombinant protein.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

16-21

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

26 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:5,000

  • ELISA

  • 1:10,000-1:20,000

TARGET

PROTEIN NAME

GST

SYNONYMS

Glutathione S Transferase antibody; Glutathione S-transferase class-mu 26 kDa isozyme antibody; GST antibody; Sj26 antigen antibody; SjGST antibody

FUNCTION

The glutathione S-transferase (GST) family of enzymes comprises a long list of cytosolic, mitochondrial, and microsomal proteins that are capable of multiple reactions with a multitude of substrates, both endogenous and xenobiotic. Mammalian cytosolic GSTs are dimeric both subunits being from the same class of GSTs. The monomers are in the range of 22–29 kDa. Glutathione S-transferase is used to create the “GST gene fusion system” in genetic engineering. Here, GST is used to purify and detect proteins of interest.

CITATIONS

  • Xian Zhao;Jianxiu Yu

    BAP1 suppresses prostate cancer progression by deubiquitinating and stabilizing PTEN

  • Deng, T., Hu, B., Wang, X.,...

    DeSUMOylation of Apoptosis Inhibitor 5 by Avibirnavirus VP3 Supports Virus Replication

  • Zhao, Qian et al.

    Dissecting the precise role of H3K9 methylation in crosstalk with DNA maintenance methylation in mammals. | Nature Communications [2016]

  • Wang, M., Ma, T., Wang, H.,...

    The RNA binding protein FgRbp1 regulates specific pre-mRNA splicing via interacting with U2AF23 in Fusarium. Nature communications, 12(1), 2661.

  • Min Liao

    Heat Shock Protein Member 8 Is an Attachment Factor for Infectious Bronchitis Virus

  • Yin, Yanni et al.

    The MAPK kinase BcMkk1 suppresses oxalic acid biosynthesis via impeding phosphorylation of BcRim15 by BcSch9 in Botrytis cinerea. | PloS Pathogens [2018]