GSK3 beta Recombinant Rabbit Monoclonal Antibody [SY28-03] (ET1607-71)
Catalog# ET1607-71
Rabbit Monoclonal to GSK3 beta
-
WB
-
IF-Cell
-
IF-Tissue
-
IHC-P
-
FC
-
IHC-Fr
-
Human
-
Mouse
-
Rat
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: A549 cell lysate
Lane 7: HepG2 cell lysate
Lane 8: MCF7 cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 40 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1607-71) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GSK3 beta Recombinant Rabbit Monoclonal Antibody [SY28-03] (ET1607-71)
Immunogen
Synthetic peptide within human gsk3 beta aa 360-400.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Jurkat cell lysate, A549 cell lysate, HepG2 cell lysate, MCF7 cell lysate, Hela, C2C12 cell lysate, C6 cell lysate, PC-3M, SKOV-3, human breast tissue, human breast carcinoma tissue, mouse testis tissue, mouse prostate tissue, mouse hippocampus tissue, mouse cerebral cortex tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY28-03
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
-
IF-Cell
-
1:50-1:200
-
IF-Tissue
-
1:50-1:200
-
IHC-P
-
1:50-1:1,000
-
FC
-
1:50-1:100
-
IHC-Fr:1:100
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 47 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GSK3 beta
SYNONYMS
Glycogen Synthase Kinase 3 Beta antibody;Glycogen synthase kinase-3 beta antibody;GSK 3 beta antibody;GSK-3 beta antibody;GSK3B antibody;GSK3B_HUMAN antibody;GSK3beta isoform antibody;Serine/threonine-protein kinase GSK3B antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. GSK-3 subfamily.
TISSUE SPECIFICITY
Expressed in testis, thymus, prostate and ovary and weakly expressed in lung, brain and kidney. Colocalizes with EIF2AK2/PKR and TAU in the Alzheimer disease (AD) brain.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by AKT1 and ILK1. Upon insulin-mediated signaling, the activated PKB/AKT1 protein kinase phosphorylates and desactivates GSK3B, resulting in the dephosphorylation and activation of GYS1. Activated by phosphorylation at Tyr-216. Inactivated by phosphorylation at Ser-9 (Probable). Phosphorylated in a circadian manner in the hippocampus (By similarity).; Mono-ADP-ribosylation by PARP10 negatively regulates kinase activity.
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Cell membrane.
FUNCTION
Glycogen synthase kinase 3 beta, also known as GSK3B, is an enzyme that in humans is encoded by the GSK3B gene. Abnormal regulation and expression of GSK3β is associated with an increased susceptibility towards bipolar disorder. Glycogen synthase kinase-3 (GSK-3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and an inactivating agent of glycogen synthase. Two isoforms, alpha (GSK3A) and beta, show a high degree of amino acid homology. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. It might be a new therapeutic target for ischemic stroke.
CITATIONS
-
Yangfan Liu , Yu Sun , Jin Yang ,et al
DNMT1-targeting remodeling global DNA hypomethylation for enhanced tumor suppression and circumvented toxicity in oral squamous cell carcinoma
-
WB
-
Human
-
Citation
-
-
Yun, X., Fang, Y., Lv, C., Qiao, S., Tao, Y., Dai, Y., & Xia, Y.
Inhibition of the activation of γδT17 cells throughPPARγ–PTEN/Akt/GSK3β/NFAT pathwaycontributes to the anti-colitis effect of madecassicacid
-
WB
-
Mouse
-
Citation
-
-
Xu, F., Zhang, J., Ji, L., Cui, W., Cui, J., Tang, Z., Sun, N., Zhang, G., Guo, M., Liu, B., & Dong, J.
Inhibition of Non-small Cell Lung Cancer by Ferroptosis and Apoptosis Induction through P53 and GSK-3β/Nrf2 Signal Pathways using Qingrehuoxue Formula
-
WB
-
Mouse
-
Citation
-
-
Jian Li; Caiqiao Zhang
Metformin Prevents Follicular Atresia in Aging Laying Chickens through Activation of PI3K/AKT and Calcium Signaling Pathways
-
WB
-
IF
-
IHC
-
hen
-
Citation
-
-
Junhao Huang,et al
The volatile oil of Acorus tatarinowii Schott ameliorates Alzheimer's disease through improving insulin resistance via activating the PI3K/AKT pathway
-
WB
-
mice
-
Citation
-
-
Shang, K., Ma, N., Che, J., Li, H., Hu, J., Sun, H., & Cao, B.
SLC27A2 mediates FAO in colorectal cancer through nongenic crosstalk regulation of the PPARs pathway
-
WB
-
Human
-
Citation
-
-
Guodong Chen;Longquan Shao
Rapamycin-Induced Autophagy Promotes the Chondrogenic Differentiation of Synovium-Derived Mesenchymal Stem Cells in the Temporomandibular Joint in Response to IL-1β
-
WB
-
Human
-
Citation
-
-
Haoyan Wu,et al
A Multifunctional miRNA Delivery System Based on Tetrahedral Framework Nucleic Acids for Regulating Inflammatory Periodontal Ligament Stem Cells and Attenuating Periodontitis Bone Loss
-
WB
-
Rat
-
Citation
-
-
Li Caixia,et al
Fucoidan ameliorates diabetic skeletal muscle atrophy through PI3K/Akt pathway
-
WB
-
Rat
-
Citation
-
-
Xue, F., Zhao, Z., Gu, Y., Han, J., Ye, K., & Zhang, Y.
7,8-Dihydroxyflavone modulates bone formation and resorption and ameliorates ovariectomy-induced osteoporosis. eLife, 10, e64872.
-
WB
-
Rat
-
Citation
-
-
Shu, Y., Zou, C., Cai, Y., He, Q., Wu, X., Zhu, H., Qv, M., Chao, Y., Xu, C., Tang, L., & Wu, X.
Vitamin C deficiency induces hypoglycemia and cognitive disorder through S-nitrosylation-mediated activation of glycogen synthase kinase 3β
-
WB
-
Mouse
-
Citation
-
-
Antileukemic effect of caffeic acid 3, 4-dihydroxyphenetyl ester. Evidences for its mechanisms of action
-
WB
-
Human
-
Citation
-
-
Gao, L., Lu, Y., Chen, H. N., Li, Z., Hu, M., Zhang, R., Wang, X., Xu, Z., Gong, Y., Wang, R., Du, D., Hai, S., Li, S., Su, D., Li, Y., Xu, H., Zhou, Z. G., & Dai, L.
Deciphering the Clinical Significance and Kinase Functions of GSK3α in Colon Cancer by Proteomics and Phosphoproteomics
-
WB
-
Human
-
Citation
-
