GSDMD Mouse Monoclonal Antibody [A8D8] (HA601047)
Catalog# HA601047
Mouse Monoclonal to GSDMD
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WB
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Human
Lane 1: PC-3 cell lysate
Lane 2: SiHa cell lysate
Lane 3: THP-1 cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 2 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601047) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
GSDMD Mouse Monoclonal Antibody [A8D8] (HA601047)
Immunogen
Recombinant protein within human gsdmd aa 101-300/484.
Host
Mouse
Positive Control
PC-3 cell lysate, SiHa cell lysate, THP-1 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A8D8
RRID
APPLICATION DILUTION
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WB
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 53 kDa
Isotype
IgG2b
TARGET
UNIPROT #
PROTEIN NAME
GSDMD
SYNONYMS
1810036L03Rik antibody;DF 5L antibody;DF5L antibody;DFNA 5L antibody;DFNA5L antibody;FKSG 10 antibody;FKSG10 antibody;FLJ12150 antibody;Gasdermin antibody;Gasdermin D antibody;Gasdermin domain containing 1 antibody;Gasdermin domain containing protein 1 antibody;Gasdermin domain-containing protein 1 antibody;Gasdermin-D antibody;GasderminD antibody;GSDMD antibody;GSDMD_HUMAN antibody;GSDMDC 1 antibody;GSDMDC1 antibody
SUBCELLULAR LOCATION
Cytosol, Inflammasome; Secreted, Cell membrane.
FUNCTION
Gasdermin D (GSDMD) is a protein that in humans is encoded by the GSDMD gene on chromosome 8. It belongs to the gasdermin family which is conserved among vertebrates and comprises six members in humans, GSDMA, GSDMB, GSDMC, GSDMD, GSDME (DFNA5) and DFNB59 (Pejvakin). Members of the gasdermin family are expressed in a variety of cell types including epithelial cells and immune cells. GSDMA, GSDMB, GSDMC, GSDMD and GSDME have been suggested to act as tumour suppressors. Several current studies have revealed that GSDMD serves as a specific substrate of inflammatory caspases (caspase-1, -4, -5 and -11) and as an effector molecule for the lytic and highly inflammatory form of programmed cell death known as pyroptosis. Hence, GSDMD is an essential mediator of host defence against microbial infection and danger signals. The pore-forming activity of the N-terminal cleavage product causes cell swelling and lysis to prevent intracellular pathogens from replicating, and is required for the release of cytoplasmic content such as the inflammatory cytokine interleukin-1β (IL-1β) into the extracellular space to recruit and activate immune cells to the site of infection. GSDMD has an additional potential role as an antimicrobial by binding to cardiolipin (CL) and form pores on bacterial membranes. Mutation of GSDMD is associated with various genetic diseases and human cancers, including brain, breast, lung, urinary bladder, cervical, skin, oral cavity, pharynx, colon, liver, cecum, stomach, pancreatic, prostate, oesophageal, head and neck, hematologic, thyroid and uterine cancers.
CITATIONS
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GLP-1RAs inhibit the activation of the NLRP3 inflammasome signaling pathway to regulate mouse renal podocyte pyroptosis
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WB
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Mouse
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Citation
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