GRP94 Mouse Monoclonal Antibody [F1-D10-G3] (M1701-12)
Catalog# M1701-12
Mouse Monoclonal to GRP94
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HepG2 cell lysate (10 µg/Lane)
Lane 2: HeLa cell lysate (10 µg/Lane)
Lane 3: Human brain tissue lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (10 µg/Lane)
Lane 5: Mouse placenta tissue lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Lane 7: PC-12 cell lysate (10 µg/Lane)
Lane 8: Rat placenta tissue lysate (20 µg/Lane)
Lane 9: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 92 kDa
Observed band size: 94 kDa
Exposure time: 25 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1701-12) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
GRP94 Mouse Monoclonal Antibody [F1-D10-G3] (M1701-12)
Immunogen
Recombinant protein within human grp94 aa 201-400 / 803.
Host
Mouse
Positive Control
HepG2 cell lysate, HeLa cell lysate, human brain tissue lysate, NIH/3T3 cell lysate, mouse placenta tissue lysate, mouse brain tissue lysate, PC-12 cell lysate, rat placenta tissue lysate, rat brain tissue lysate, human liver cancer tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
F1-D10-G3
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:10,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 94 kDa
Isotype
IgM
TARGET
UNIPROT #
PROTEIN NAME
GRP94
SYNONYMS
94 kDa glucose regulated protein antibody;94 kDa glucose-regulated protein antibody;ECGP antibody;Endoplasmin antibody;Endothelial cell (HBMEC) glycoprotein antibody;ENPL_HUMAN antibody;Glucose regulated protein 94kDa antibody;gp96 antibody;gp96 homolog antibody;GRP 94 antibody;GRP-94 antibody;Heat shock protein 90 kDa beta member 1 antibody;heat shock protein 90kDa beta (Grp94), member 1 antibody;Heat shock protein, 90 kDa, beta, 1 antibody;HSP90B1 antibody;Stress inducible tumor rejection antigen GP96 antibody;TRA1 antibody;tumor rejection antigen (gp96) 1 antibody;Tumor rejection antigen 1 antibody;Tumor rejection antigen gp96 antibody;Tumor rejection antigen-1 (gp96) antibody
SEQUENCE SIMILARITIES
Belongs to the heat shock protein 90 family.
SUBCELLULAR LOCATION
Endoplasmic reticulum lumen, melanosome.
FUNCTION
The human HSP 90 family includes 17 genes that fall into four classes: HSP90AA, HSP90AB, HSP90B and TRAP. HSP 90 family members guide the normal folding, intracellular disposition and Proteolytic turnover of many key regulators of cell growth, differentiation and survival. HSP 90α, also designated HSP90A, HSP 86 and LPS-associated protein 2 (LAP2), is a cytosolic enhancer of inducible nitric-oxide synthase (iNOS), with chaperone activity that is important for the transcriptional activity of p53. HSP 90β, also designated HSP90B, HSP 84 and HSPC2, is a cytosolic protein that participates in signaling pathways with PKC e to protect cells from external damage, particularly in heat shock-mediated events. GRP 94, also known as tumor rejection antigen 1 (TRA1), ECGP and GP96, localizes to the ER, is highly expressed in BGC-823 human gastric carcinoma cells and is upregulated in human endothelial cells in response to hypoxia by HIF-1.
CITATIONS
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Wu, H., Guo, H., Liu, H., Cui, H., Fang, J., Zuo, Z., Deng, J., Li, Y., Wang, X., & Zhao, L.
Copper sulfate-induced endoplasmic reticulum stress promotes hepatic apoptosis by activating CHOP, JNK and caspase-12 signaling pathways
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WB
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Mouse
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Citation
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Jinliang Nan,Yinuo Lin
Lipofectamine 2000/siRNA complexes cause endoplasmic reticulum unfolded protein response in human endothelial cells. Journal of cellular physiology, 234(11), 21166–21181.
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WB
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IF
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Human coronary artery endothelial cells (HCAECs)
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Citation
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