GRP78 / BIP Recombinant Mouse Monoclonal Antibody [C9-9-R] (HA601076)
Catalog# HA601076
Mouse Monoclonal to GRP78 / BIP
-
WB
-
IHC-P
-
IF-Cell
-
Human
-
Mouse
-
Rat
-
Zebrafish
Western blot analysis of GRP78 / BIP on different lysates with Mouse anti-GRP78 / BIP antibody (HA601076) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate (15 µg/Lane)
Lane 2: HepG2 cell lysate (15 µg/Lane)
Lane 3: Mouse brain tissue lysate (30 µg/Lane)
Lane 4: U-87 MG cell lysate (30 µg/Lane)
Lane 5: RAW264.7 cell lysate (30 µg/Lane)
Lane 6: RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate (30 µg/Lane)
Lane 7: Mouse liver tissue lysate (30 µg/Lane)
Lane 8: Rat liver tissue lysate (30 µg/Lane)
Lane 9: Rat pancreas tissue lysate (30 µg/Lane)
Predicted band size: 72 kDa
Observed band size: 72 kDa
Exposure time: Lane 1-3: 11 seconds; Lane 4-9: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601076) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Mouse monoclonal primary
Product Name
GRP78 / BIP Recombinant Mouse Monoclonal Antibody [C9-9-R] (HA601076)
Immunogen
Recombinant protein within human grp78 aa 100-400.
Host
Mouse
Positive Control
MCF7 cell lysate, HepG2 cell lysate, mouse brain tissue lysate, U-87 MG cell lysate, RAW264.7 cell lysate, RAW264.7 treated with 300nM Thapsigargin for 18 hours cell lysate, mouse liver tissue lysate, rat liver tissue lysate, rat pancreas tissue lysate, rat brain tissue, mouse small intestine tissue, Hela.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
C9-9-R
RRID
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
IF-Cell
-
1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 72 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
GRP78 / BIP
SYNONYMS
78 kDa glucose regulated protein antibody;78 kDa glucose-regulated protein antibody;AL022860 antibody;AU019543 antibody;BIP antibody;D2Wsu141e antibody;D2Wsu17e antibody;Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody;Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody;Epididymis secretory sperm binding protein Li 89n antibody;FLJ26106 antibody;Glucose Regulated Protein 78 antibody;Glucose Regulated Protein 78kDa antibody;GRP 78 antibody;GRP-78 antibody;GRP78 antibody;GRP78_HUMAN antibody;Heat shock 70 kDa protein 5 antibody;Heat Shock 70kDa Protein 5 antibody;Heat shock protein family A (Hsp70) member 5 antibody;HEL S 89n antibody;Hsce70 antibody;HSPA 5 antibody;HSPA5 antibody;Immunoglobulin Heavy Chain Binding Protein antibody;Immunoglobulin heavy chain-binding protein antibody;mBiP antibody;MIF2 antibody;Sez7 antibody
SUBCELLULAR LOCATION
Cytoplasm, endoplasmic reticulum lumen
FUNCTION
Endoplasmic reticulum chaperone that plays a key role in protein folding and quality control in the endoplasmic reticulum lumen. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10/ERdj5, probably to facilitate the release of DNAJC10/ERdj5 from its substrate. Acts as a key repressor of the ERN1/IRE1-mediated unfolded protein response. In the unstressed endoplasmic reticulum, recruited by DNAJB9/ERdj4 to the luminal region of ERN1/IRE1, leading to disrupt the dimerization of ERN1/IRE1, thereby inactivating ERN1/IRE1. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP from ERN1/IRE1, allowing homodimerization and subsequent activation of ERN1/IRE1. Plays an auxiliary role in post-translational transport of small presecretory proteins across endoplasmic reticulum (ER). May function as an allosteric modulator for SEC61 channel-forming translocon complex, likely cooperating with SEC62 to enable the productive insertion of these precursors into SEC61 channel. Appears to specifically regulate translocation of precursors having inhibitory residues in their mature region that weaken channel gating. May also play a role in apoptosis and cell proliferation. Plays an important role in viral binding to the host cell membrane and entry for several flaviruses such as Dengue virus, Zika virus and Japanese encephalitis virus. Acts as a component of the cellular receptor for Dengue virus serotype 2/DENV-2 on human liver cells. Acts as a receptor for CotH proteins expressed by fungi of the order mucorales, the causative agent of mucormycosis, which plays an important role in epithelial cell invasion by the fungi. Acts as a receptor for R.delemar CotH3 in nasal epithelial cells, which may be an early step in rhinoorbital/cerebral mucormycosis (RCM) disease progression.
