Grp75 Mouse Monoclonal Antibody [6-A9] (EM1701-23)
Catalog# EM1701-23
Mouse Monoclonal to Grp75
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WB
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate
Lane 2: K-562 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: Mouse liver tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Rat kidney tissue lysate
Predicted band size: 74 kDa
Observed band size: 74 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-23) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
Grp75 Mouse Monoclonal Antibody [6-A9] (EM1701-23)
Immunogen
Synthetic peptide within human grp75 aa 101-150.
Host
Mouse
Positive Control
HepG2 cell lysate, L929 cell lysate, K562 cell lysate, NIH-3T3 cell lysate, A549, HepG2, SK-Br-3, rat epididymis tissue, mouse liver tissue, mouse oviduct tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
6-A9
RRID
APPLICATION DILUTION
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WB
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1:500-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 74 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
Grp75
SYNONYMS
75 kDa glucose regulated protein antibody;75 kDa glucose-regulated protein antibody;CSA antibody;Glucose Regulated Protein antibody;Grp 75 antibody;GRP-75 antibody;GRP75 antibody;GRP75_HUMAN antibody;Heat shock 70 kDa protein 9 antibody;Heat shock 70kD protein 9 antibody;heat shock 70kDa protein 9 antibody;Heat shock 70kDa protein 9B antibody;Heat shock protein 74 kDa A antibody;Heat shock protein A antibody;Heat shock protein cognate 74 antibody;Hsc74 antibody;Hsp74 antibody;Hsp74a antibody;HSPA9 antibody;Hspa9a antibody;HSPA9B antibody;MGC4500 antibody;mitochondrial antibody;Mortalin 2 antibody;Mortalin antibody;Mortalin perinuclear antibody;Mortalin2 antibody;MOT 2 antibody;MOT antibody;MOT2 antibody;Mthsp70 antibody;p66 mortalin antibody;P66 MOT antibody;PBP74 antibody;Peptide binding protein 74 antibody;Peptide-binding protein 74 antibody;Stress 70 protein mitochondrial antibody;Stress 70 protein mitochondrial precursor antibody;Stress-70 protein antibody
SEQUENCE SIMILARITIES
Belongs to the heat shock protein 70 family.
SUBCELLULAR LOCATION
Mitochondrion. Nucleus.
FUNCTION
The HSP 70 family comprises four highly conserved proteins, HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. HSC 70, GRP 75 and GRP 78 are constitutively expressed in primate cells. HSP 70 expression is strongly induced in response to heat stress. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 expression is restricted to the mitochondrial matrix and aids in the translocation and folding of nascent polypeptide chains of both nuclear and mitochondrial origin. GRP 75 and GRP 78 are unresponsive to heat stress and are induced by glucose deprivation.
CITATIONS
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Hai-bo Qiu
Ang II-AT2R increases mesenchymal stem cell migration by signaling through the FAK and RhoA/Cdc42 pathways in vitro
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WB
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Human
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Citation
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