GRB2 Mouse Monoclonal Antibody [11-B11] (EM1701-41)
Catalog# EM1701-41
Mouse Monoclonal to GRB2
Tested for
-
WB
-
IHC-P
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IF-Cell
Reacts with
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Human
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Mouse
-
Rat
Western blot analysis of GRB2 on different lysates with Mouse anti-GRB2 antibody (EM1701-41) at 1/1,000 dilution.
Lane 1: 293T cell lysate (30 µg/Lane)
Lane 2: A431 cell lysate (30 µg/Lane)
Lane 3: NIH/3T3 cell lysate (30 µg/Lane)
Lane 4: PC-12 cell lysate (30 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: 293T cell lysate (30 µg/Lane)
Lane 2: A431 cell lysate (30 µg/Lane)
Lane 3: NIH/3T3 cell lysate (30 µg/Lane)
Lane 4: PC-12 cell lysate (30 µg/Lane)
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (EM1701-41) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.