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PRODUCT CODE: EM1701-41

GRB2 Mouse Monoclonal Antibody [11-B11] (EM1701-41)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.
  • Western blot analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.
  • ICC staining GRB2 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GRB2 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GRB2 antibody. Counter stained with hematoxylin.
Western blot analysis of GRB2 on 293 cell lysate using anti-GRB2 antibody at 1/1,000 dilution.

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GRB2 Mouse Monoclonal Antibody [11-B11] (EM1701-41)

Immunogen

Recombinant protein within human grb2 aa 21-217 / 217.

Host

Mouse

Positive Control

293, MCF-7, HepG2, rat brain tissue, human colon cancer tissue,

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

11-B11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P62993

PROTEIN NAME

GRB2

SYNONYMS

Abundant SRC homology antibody; Adapter protein GRB2 antibody; ASH antibody; Ash protein antibody; EGFRBP GRB2 antibody; Epidermal growth factor receptor binding protein antibody; Epidermal growth factor receptor binding protein GRB2 antibody; GRB 2 antibody; GRB2 adapter protein antibody; Grb2 antibody; GRB2_HUMAN antibody; Grb3 3 antibody; Growth factor receptor bound protein 2 antibody; Growth factor receptor bound protein 3 antibody; Growth factor receptor-bound protein 2 antibody; HT027 antibody; MST084 antibody; MSTP084 antibody; NCKAP2 antibody; OTTHUMP00000166096 antibody; OTTHUMP00000166097 antibody; OTTHUMP00000166098 antibody; Protein ash antibody; SEM5 antibody; SH2/SH3 adapter GRB2 antibody

SEQUENCE SIMILARITIES

Belongs to the GRB2/sem-5/DRK family.

SUBCELLULAR LOCATION

Endosome, Nucleus, Golgi apparatus.

FUNCTION

Growth factor receptor-bound protein 2 also known as Grb2 is an adaptor protein involved in signal transduction/cell communication. In humans, the GRB2 protein is encoded by the GRB2 gene. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway.