GRAMD1A Mouse Monoclonal Antibody [1-E4] (M1010-3)
Catalog# M1010-3
Mouse Monoclonal to GRAMD1A
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
Specifications
Product Type
Mouse monoclonal primary
Product Name
GRAMD1A Mouse Monoclonal Antibody [1-E4] (M1010-3)
Immunogen
Recombinant protein within human gramd1a aa 1-724 / 724.
Host
Mouse
Positive Control
NIH/3T3 cells lysates, SHG-44 cells lysates, A549, human liver tissue, human liver carcinoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
1-E4
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:50-1:200
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IHC-P
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1:50-1:400
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 81 kDa
Isotype
IgM
TARGET
UNIPROT #
PROTEIN NAME
GRAMD1A
TISSUE SPECIFICITY
Expressed in liver.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane, Cell membrane, Cytoplasmic vesicle, autophagosome.
FUNCTION
GRAMD1A first finds in human embryonic stem cell, and expresses in ectoderm, mesoderm and endoderm tissues and many tumor cells, its function hasn’t been explored. The researh has determined the role of GRAM1DA in the self-renewal of HCC stem cell and resistance to chemotherapy by overexpressing GRAMD1A, and the role of GRAMD1A in tumor growth by overexpressing or downregulating GRAMD1A. GRAMD1A is a prognostic factor for HCC, it promoted the self-renewal of HCC stem cell, resistance to chemotherapy and tumor growth through promoting STAT5, Inhibition of STAT5 in indicated cells with GRAMD1A overexpression inhibited the self-renewal of HCC stem cell, resistance to chemotherapy and tumor growth.
CITATIONS
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Chen, L. J., Zhang, N. N., Zhou, C. X., Yang, Z. X., Li, Y. R., Zhang, T., Li, C. R., Wang, X., Wang, Y., Wang, Z. B., Xia, Z. R., Wang, Z. B., Zhang, C. L., Guan, Y. C., Sun, Q. Y., & Zhang, D.
Gm364 coordinates MIB2/DLL3/Notch2 to regulate female fertility through AKT activation
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IP
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Citation
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