GPX4 Recombinant Rabbit Monoclonal Antibody [JU11-31] (ET1706-45)
Catalog# ET1706-45
Rabbit Monoclonal to GPX4
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WB
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IHC-P
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IF-Cell
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FC
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Human
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Mouse
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Rat
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Zebrafish
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-GPX4 antibody (ET1706-45) at 1/200 dilution and competitor's antibody at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GPX4 Recombinant Rabbit Monoclonal Antibody [JU11-31] (ET1706-45)
Immunogen
Synthetic peptide within human gpx4 aa 23-72 / 197.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, HepG2 cell lysate, U-937 cell lysate, HEK-293 cell lysate, mouse testis tissue lysate, rat testis tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, zebrafish tissue lysates, HEK-293, rat epididymis tissue, human kidney tissue, mouse testis tissue, MCF-7, PC-3M, LOVO.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU11-31
RRID
APPLICATION DILUTION
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WB
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1:10,000
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IHC-P
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1:200-1:1,000
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IF-Cell
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1:50-1:200
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FC:1
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10,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 22 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GPX4
SYNONYMS
Glutathione peroxidase 4 antibody;GPX 4 antibody;GPX-4 antibody;GPX4 antibody;GPX4_HUMAN antibody;GSHPx-4 antibody;MCSP antibody;mitochondrial antibody;PHGPx antibody;Phospholipid hydroperoxidase antibody;Phospholipid hydroperoxide glutathione peroxidase antibody;Phospholipid hydroperoxide glutathione peroxidase mitochondrial antibody;snGPx antibody;snPHGPx antibody;Sperm nucleus glutathione peroxidase antibody
SEQUENCE SIMILARITIES
Belongs to the glutathione peroxidase family.
TISSUE SPECIFICITY
Present primarily in testis. Expressed in platelets (at protein level).
SUBCELLULAR LOCATION
Mitochondrion, Cytoplasm.
FUNCTION
Glutathione peroxidase (GPx) enzymes are generally selenium-containing tetrameric glycoproteins that help prevent lipid peroxidation of cell membranes. GPx enzymes reduce lipid hydroperoxides to alcohols, and reduce free hydrogen peroxide to water. GPx members are among the few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA. There are eight GPx homologs (GPx-1-8). GPx-1, Gpx-2 and Gpx-3 exist as homotetramers. Gpx-4 has a high tendancy to form high molecular weight oligomers.
CITATIONS
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Xiao Juan,et al
Icariin inhibits chondrocyte ferroptosis and alleviates osteoarthritis by enhancing the SLC7A11/GPX4 signaling
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WB
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IHC
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Rat
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Human
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Citation
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Sun Yonghua,et al
An intestinal DHA-PA-PG axis promotes digestive organ expansion by mediating usage of maternally-deposited yolk lipids
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IF
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WB
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Mouse
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Citation
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Jia Degong,et al
Wogonin Alleviates DCD Liver Ischemia/Reperfusion Injury by Regulating ALOX15/iNOS-mediated Ferroptosis
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WB
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Rat
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Citation
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-
Wu, L., Ishigaki, Y., Zeng, W., Harimoto, T., Yin, B., Chen, Y., Liao, S., Liu, Y., Sun, Y., Zhang, X., Liu, Y., Liang, Y., Sun, P., Suzuki, T., Song, G., Fan, Q., & Ye, D.
Generation of hydroxyl radical-activatable ratiometric near-infrared bimodal probes for early monitoring of tumor response to therapy
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WB
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Citation
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Agrimonolide inhibits cancer progression and induces ferroptosis and apoptosis by targeting SCD1 in ovarian cancer cells
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WB
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Human
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Citation
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-
He Yijing,et al
Polystyrene nanoplastic exposure actives ferroptosis by oxidative stress-induced lipid peroxidation in porcine oocytes during maturation
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WB
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Porcine
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Citation
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-
Canonical Wnt signaling works downstream of iron overload to prevent ferroptosis from damaging osteoblast differentiation
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WB
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Mouse
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Citation
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-
Lu Feng,et al
QBT improved cognitive dysfunction in rats with vascular dementia by regulating the Nrf2/xCT/GPX4 and NLRP3/Caspase-1/GSDMD pathways to inhibit ferroptosis and pyroptosis of neurons
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WB
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Citation
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Qin, L. Y., Guan, P., Wang, J. X., Chen, Y., Zhao, Y. S., Yang, S. C., Guo, Y. J., Wang, N., & Ji, E. S.
Therapeutic Potential of Astragaloside IV Against Adriamycin-Induced Renal Damage in Rats via Ferroptosis
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IHC
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WB
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Rat
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Citation
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-
Li L, Ye Z, Xia Y, et al
YAP/ACSL4 Pathway-Mediated Ferroptosis Promotes Renal Fibrosis in the Presence of Kidney Stones
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WB
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Mouse
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Citation
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-
Jicheng Jiang,et al
Nanovesicle-templated nanogel: A dual-modal platform integrating intracellular iron homeostasis disruption for ferroptosis and MRI-guided tumor diagnosis
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WB
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Mouse
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Citation
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Wu Ziying,et al
Multimodal enhancement of ferroptosis for synergistic cascade colorectal cancer therapy
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IF
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Mouse
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Citation
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-
Chen Hao,et al
Vitamin D inhibits ferroptosis and mitigates the kidney injury of prediabetic mice by activating the Klotho/p53 signaling pathway
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WB
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Mouse
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Citation
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Sun, H., Wang, D., Ren, J., Liu, J., Wang, Z., Wang, X., Zhang, A., Yang, K., Yang, M., & Zhou, H.
Vitamin D ameliorates Aeromonas hydrophila-induced iron-dependent oxidative damage of grass carp splenic macrophages by manipulating Nrf2-mediated antioxidant pathway
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WB
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Yellow catfish
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Citation
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-
Zhao, J., Xu, B., Xiong, Q., Feng, Y., & Du, H.
Erastin-induced ferroptosis causes physiological and pathological changes in healthy tissues of mice
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WB
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Mouse
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Citation
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-
Deng Yuxin,et al
A novel apoferritin nanocage with ECM promoting, ferroptosis suppressing and inflammation targeting property for osteoarthritis therapy
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WB
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Mouse
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Citation
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-
Nahua Xu,et al
Reserpine alleviates cisplatin-induced acute kidney injury via anti-ferroptosis and cGAS/STING pathway
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WB
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Rat
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Human
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Citation
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-
Ding Aoxue,et al
Sesquiterpene lactones from Tithonia diversifolia with ferroptosis-inducing activities
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WB
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Mouse
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Citation
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-
Sun, Y., Xu, H., Tan, B., Yi, Q., Liu, H., Chen, T., Xiang, H., Wang, R., Xie, Q., Tian, J., & Zhu, J.
Andrographolide protects bone marrow mesenchymal stem cells against glucose and serum deprivation under hypoxia via the NRF2 signaling pathway
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WB
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Rat
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Citation
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-
Wang Tianxiang,et al
Flavonoid 4, 4′-dimethoxychalcone selectively eliminates senescent cells via activating ferritinophagy
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WB
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Mouse
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Citation
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Song, J. X., An, J. R., Chen, Q., Yang, X. Y., Jia, C. L., Xu, S., Zhao, Y. S., & Ji, E. S.
Liraglutide attenuates hepatic iron levels and ferroptosis in db/db mice
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WB
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Mouse
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Citation
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-
p53 deacetylation alleviates calcium oxalate deposition-induced renal fibrosis by inhibiting ferroptosis
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WB
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Mouse
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Citation
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Xie, Q., Sun, Y., Xu, H., Chen, T., Xiang, H., Liu, H., Wang, R., Tan, B., Yi, Q., Tian, J., & Zhu, J.
Ferrostatin-1 improves BMSC survival by inhibiting ferroptosis
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WB
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Rat
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Citation
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Ma L, Liu X, Zhang M, et al
Paeoniflorin alleviates ischemia/reperfusion induced acute kidney injury by inhibiting Slc7a11-mediated ferroptosis
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WB
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IHC
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Human
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Citation
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-
Hailei Shan,et al
Bone marrow mesenchymal stem cells with PTBP1 knockdown protect against cerebral ischemia-reperfusion injury by inhibiting ferroptosis via the JNK/P38 pathway in rats
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WB
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Citation
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Zhang Yiran,et al
Iron-Based Nanovehicle Delivering Fin56 for Hyperthermia-Boosted Ferroptosis Therapy Against Osteosarcoma
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IF
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Mouse
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Citation
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-
Boosting ferroptosis and microtubule inhibition for antitumor therapy via a carrier-free supermolecule nanoreactor
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WB
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mouse
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Citation
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-
Shikonin and cisplatin synergistically overcome cisplatin resistance of ovarian cancer by inducing ferroptosis via upregulation of HMOX1 to promote Fe2+ accumulation
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WB
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Human
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Citation
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-
ROS-responsive fluorinated polyethyleneimine vector to co-deliver shMTHFD2 and shGPX4 plasmids induces ferroptosis and apoptosis for cancer therapy
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WB
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Mouse
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Citation
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Chen Ying,et al
Cpd-A1 alleviates acute kidney injury by inhibiting ferroptosis
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WB
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Mouse
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Citation
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-
Cyclovirobuxine D pretreatment ameliorates septic heart injury through mitigation of ferroptosis
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WB
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Rat
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Citation
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-
Meijiang Zhu,et al
Salidroside alleviates ferroptosis in FAC-induced Age-related macular degeneration models by activating Nrf2/SLC7A11/GPX4 axis
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WB
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Mouse
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Citation
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-
Bao Jiaqi,et al
Effects of the ferroptosis inducer erastin on osteogenic differentiation and biological pathways of primary osteoblasts
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WB
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Human
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Citation
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-
Zhang Yiyuan,et al
SBFI26 induces triple- negative breast cancer cells ferroptosis via lipid peroxidation
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WB
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Human
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Citation
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-
Protective effect of rutin on ferroptosis‐induced oxidative stress in aging laying hens through Nrf2/HO‐1 signaling
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WB
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Rutin
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Citation
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-
Huang, Y., Zhang, N., Xie, C., You, Y., Guo, L., Ye, F., Xie, X., & Wang, J.
Lipocalin-2 in neutrophils induces ferroptosis in septic cardiac dysfunction via increasing labile iron pool of cardiomyocytes
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WB
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Mouse
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Citation
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-
Zhong Xin,et al
Angiotension II directly bind P2X7 receptor to induce myocardial ferroptosis and remodeling by activating human antigen R
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WB
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Mouse
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Citation
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-
Aiyuan Zhou,et al
Loss of interferon regulatory factor-1 prevents lung fibrosis by upregulation of pon1 expression
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WB
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Mouse
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Citation
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-
Huang Z , Dai L, Peng F,et al
mTOR signaling pathway regulates embryonic development and rapid growth of triploid crucian carp
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IHC
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Mouse
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Citation
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-
Yang, Q., Zuo, Z., Zeng, Y., Ouyang, Y., Cui, H., Deng, H., Zhu, Y., Deng, J., Geng, Y., Ouyang, P., Lai, W., Du, Z., Ni, X., Yin, H., Fang, J., & Guo, H.
Autophagy-mediated ferroptosis involved in nickel-induced nephrotoxicity in the mice
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WB
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Mouse
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Citation
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-
Ouyang, Z. Q., Shao, L. S., Wang, W. P., Ke, T. F., Chen, D., Zheng, G. R., Duan, X. R., Chu, J. X., Zhu, Y., Yang, L., Shan, H. Y., Huang, L., & Liao, C. D.
Low intensity pulsed ultrasound ameliorates Adriamycin-induced chronic renal injury by inhibiting ferroptosis
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WB
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Rat
-
Citation
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-
Ma Yajun,et al
Closed‐loop of Catalyzed Lactate Oxidization Synergistically Sensitizes Apoptosis and Ferroptosis for Cancer Therapy
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WB
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Mouse
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Citation
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-
Congcong Lu,et al
Shikonin induces ferroptosis in osteosarcomas through the mitochondrial ROS-regulated HIF-1α/HO-1 axis
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IHC
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Mouse
-
Citation
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-
Bao J, Wang Z, Yang Y, et al
Interleukin-17 alleviates erastin-induced alveolar bone loss by suppressing ferroptosis via interaction between NRF2 and p-STAT3
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WB
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Mouse
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Citation
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-
Ruiqin Hu,et al
bmp10 maintains cardiac function by regulating iron homeostasis
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WB
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Zebrafish
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Citation
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-
Shuze Chen,et al
Periodontal pathogen Fusobacterium nucleatum promotes ulcerative colitis by ferroptosis-mediated gut barrier disruption
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WB
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Citation
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-
Li Qin,et al
DDX39B protects against sorafenib-induced ferroptosis by facilitating the splicing and cytoplasmic export of GPX4 pre-mRNA in hepatocellular carcinoma
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WB
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Human
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Citation
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-
Songyuan Yang,et al
The SIRT6/BAP1/xCT signaling axis mediates ferroptosis in cisplatin-induced AKI
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WB
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human
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Citation
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-
Danlei Zhang,et al
Exploring the Mechanisms of the Ferroptosis-Related Gene TGFBR1 in Autoimmune Uveitis Based on Machine Learning Models
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WB
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mice
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Citation
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-
Boosting ferroptosis and microtubule inhibition for antitumor therapy via a carrier-free supermolecule nanoreactor
-
WB
-
Mouse
-
Citation
-
-
Jia Cuiling,et al
Rosmarinic acid liposomes suppress ferroptosis in ischemic brain via inhibition of TfR1 in BMECs
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WB
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Mouse
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Citation
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-
Sun, Y., He, Y., Tong, J., Liu, D., Zhang, H., He, T., & Bi, Y.
All-trans retinoic acid inhibits the malignant behaviors of hepatocarcinoma cells by regulating ferroptosis
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WB
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Human
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Citation
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-
Liang, J., Shen, Y., Wang, Y., Huang, Y., Wang, J., Zhu, Q., Tong, G., Yu, K., Cao, W., Wang, Q., Li, Y., & Zhao, Y.
Ferroptosis participates in neuron damage in experimental cerebral malaria and is partially induced by activated CD8 + T cells
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WB
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IHC
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IF
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Mouse
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Citation
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-
Guo Shanshan,et al
Baicalein alleviates cisplatin-induced acute kidney injury by inhibiting ALOX12-dependent ferroptosis
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WB
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Mouse
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Citation
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-
Liu X, Ai Y, Xiao M, et al
PM 2.5 juvenile exposure-induced spermatogenesis dysfunction by triggering testes ferroptosis and antioxidative vitamins intervention in adult male rats
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WB
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Rat
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Citation
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-
Dihydroartemisinin inhibits the growth of pancreatic cells by inducing ferroptosis and activating antitumor immunity
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WB
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Mouse
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Citation
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Jin, L., Yu, B., Wang, H., Shi, L., Yang, J., Wu, L., Gao, C., Pan, H., Han, F., Lin, W., Lai, E. Y., Wang, Y. F., & Yang, Y.
STING promotes ferroptosis through NCOA4-dependent ferritinophagy in acute kidney injury
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WB
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Human
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Mouse
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Citation
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