PRODUCT CODE: EM1701-96

GPX1 Mouse Monoclonal Antibody [A6-C0-B9] (EM1701-96)

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

Western blot analysis of Glutathione Peroxidase 1 on THP-1 cell lysate using anti- Glutathione Peroxidase 1 antibody at 1/500 dilution.
  • Western blot analysis of Glutathione Peroxidase 1 on THP-1 cell lysate using anti- Glutathione Peroxidase 1 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Glutathione Peroxidase 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.
Western blot analysis of Glutathione Peroxidase 1 on THP-1 cell lysate using anti- Glutathione Peroxidase 1 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

GPX1 Mouse Monoclonal Antibody [A6-C0-B9] (EM1701-96)

Immunogen

Recombinant protein within human glutathione peroxidase 1 aa 20-203 / 203.

Host

Mouse

Positive Control

THP-1, human liver tissue, human kidney tissue, HepG2.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A6-C0-B9

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

ProG purified.

MOLECULAR WEIGHT

22 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GPX1

SYNONYMS

AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody

SEQUENCE SIMILARITIES

Belongs to the glutathione peroxidase family.

TISSUE SPECIFICITY

Expressed in platelets (at protein level).

POST-TRANSLATIONAL MODIFICATION

During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.

SUBCELLULAR LOCATION

Cytosol. Mitochondrion.

FUNCTION

The protein encoded by this gene belongs to the glutathione peroxidase family, members of which catalyze the reduction of organic hydroperoxides and hydrogen peroxide (H2O2) by glutathione, and thereby protect cells against oxidative damage. Other studies indicate that H2O2 is also essential for growth-factor mediated signal transduction, mitochondrial function, and maintenance of thiol redox-balance; therefore, by limiting H2O2 accumulation, glutathione peroxidases are also involved in modulating these processes. Several isozymes of this gene family exist in vertebrates, which vary in cellular location and substrate specificity. This isozyme is the most abundant, is ubiquitously expressed and localized in the cytoplasm, and whose preferred substrate is hydrogen peroxide.