PRODUCT CODE: EM1701-53

Gp83 Mouse Monoclonal Antibody [4D1] (EM1701-53)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution.
  • Western blot analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution.
  • ICC staining Gp83 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Gp83 antibody. Counter stained with hematoxylin.
Western blot analysis of Gp83 on SH-SY-5Y cells lysates using anti-Gp83 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

Gp83 Mouse Monoclonal Antibody [4D1] (EM1701-53)

Immunogen

Recombinant protein with human adam17 aa 215-315 / 824.

Host

Mouse

Positive Control

SH-SY-5Y, PC-3M, rat epididymis tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

4D1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

85 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Gp83

TISSUE SPECIFICITY

Not detected in healthy melanocytes. Expressed in melanoma cells.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

This gene encodes a member of the ADAMs family of zinc proteases. These transmembrane proteins play roles in multiple processes including cell signaling, adhesion and migration. The encoded protein lacks protease activity and may play roles in protein-protein interactions and cell adhesion processes including sperm-egg fusion. Mutations in this gene may be involved in the progression of melanoma. May play an important role in male reproduction including sperm maturation and gonadotrope function. This is a non catalytic metalloprotease-like protein.