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PRODUCT CODE: ET1704-64

GOLPH2 Recombinant Rabbit Monoclonal Antibody [JA63-03] (ET1704-64)

  • Recombinant

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of GOLPH2 on SH-SY5Y cell lysate using anti-GOLPH2 antibody at 1/1,000 dilution.
  • Western blot analysis of GOLPH2 on SH-SY5Y cell lysate using anti-GOLPH2 antibody at 1/1,000 dilution.
  • ICC staining GOLPH2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GOLPH2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining GOLPH2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-GOLPH2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-GOLPH2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of Hela cells with GOLPH2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
Western blot analysis of GOLPH2 on SH-SY5Y cell lysate using anti-GOLPH2 antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GOLPH2 Recombinant Rabbit Monoclonal Antibody [JA63-03] (ET1704-64)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SH-SY5Y cell lysate, 293T, A549, Hela, human prostate tissue, mouse small intestine tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JA63-03

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

100 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50

TARGET

UNIPROT #

Q8NBJ4

PROTEIN NAME

GOLPH2

SYNONYMS

bA379P1.3 antibody; C9orf155 antibody; Chromosome 9 open reading frame 155 antibody; Golgi membrane protein 1 antibody; Golgi membrane protein GP73 antibody; Golgi phosphoprotein 2 antibody; Golgi protein 73 kD antibody; Golgi protein 73kD antibody; GOLM 1 antibody; GOLM1 antibody; GOLM1_HUMAN antibody; GOLPH 2 antibody; GOLPH2 antibody; GP 73 antibody; GP73 antibody; PSEC0257 antibody

SEQUENCE SIMILARITIES

Belongs to the GOLM family.

TISSUE SPECIFICITY

Widely expressed. Highly expressed in colon, prostate, trachea and stomach. Expressed at lower level in testis, muscle, lymphoid tissues, white blood cells and spleen. Predominantly expressed by cells of the epithelial lineage. Expressed at low level in normal liver. Expression significantly increases in virus (HBV, HCV) infected liver. Expression does not increase in liver disease due to non-viral causes (alcohol-induced liver disease, autoimmune hepatitis). Increased expression in hepatocytes appears to be a general feature of advanced liver disease. In liver tissue from patients with adult giant-cell hepatitis (GCH), it is strongly expressed in hepatocytes-derived syncytial giant cells. Constitutively expressed by biliary epithelial cells but not by hepatocytes.

POST-TRANSLATIONAL MODIFICATION

Glycosylated.; Phosphorylation sites are present in the extracellular medium.

SUBCELLULAR LOCATION

Golgi apparatus.

FUNCTION

GP73 (also known as Golgi phosphoprotein 2, GOLPH 2 or Golgi membrane protein), is a widely expressed, epithelial-specific, type II transmembrane protein which resides in the Golgi apparatus, where it is responsible for the posttranslational modification of proteins produced in the Rough ER while assisting in the transport of proteins through the Golgi. The human GP73 gene has been mapped within a BAC and localized to chromosome 9q21.33. GP73 levels rise in those who have been diagnosed with acute and chronic liver diseases.