Glutathione Synthetase Recombinant Rabbit Monoclonal Antibody [JB95-33] (ET7107-62)

UNIPROT #

P48637

PROTEIN NAME

Glutathione Synthetase

SYNONYMS

epididymis secretory sperm binding protein Li 64p antibody;epididymis secretory sperm binding protein Li 88n antibody;Glutathione synthase antibody;Glutathione synthetase antibody;GSH S antibody;GSH synthetase antibody;GSH-S antibody;GSHB_HUMAN antibody;GSHS antibody;GSS antibody;HEL-S-64p antibody;HEL-S-88n antibody;MGC14098 antibody;OTTHUMP00000030711 antibody

SEQUENCE SIMILARITIES

Belongs to the eukaryotic GSH synthase family.

SUBCELLULAR LOCATION

Cytosol. Extracellular region or secreted.

FUNCTION

GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel β-sheet, a parallel β-sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with γ-GCS and ending with GSS. In an ATP-dependent reaction, GSS produces Glutathione from γ-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamide increase the ex-pression of GSS, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GSS deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline.