PRODUCT CODE: ET7107-62

Glutathione Synthetase Recombinant Rabbit Monoclonal Antibody [JB95-33] (ET7107-62)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: SiHa<br />
  Lane 2: Mouse colon<br />
  Lane 3: Rat liver
  • Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.<br />
  Positive control:<br />
  Lane 1: SiHa<br />
  Lane 2: Mouse colon<br />
  Lane 3: Rat liver
  • ICC staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution.
Positive control:
Lane 1: SiHa
Lane 2: Mouse colon
Lane 3: Rat liver

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Glutathione Synthetase Recombinant Rabbit Monoclonal Antibody [JB95-33] (ET7107-62)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

SiHa, mouse colon tissue, rat liver tissue lysate, rat epididymis tissue, human tonsil tissue, human colon cancer tissue, human kidney tissue, HepG2, LOVO.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB95-33

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

52 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glutathione Synthetase

SYNONYMS

epididymis secretory sperm binding protein Li 64p antibody; epididymis secretory sperm binding protein Li 88n antibody; Glutathione synthase antibody; Glutathione synthetase antibody; GSH S antibody; GSH synthetase antibody; GSH-S antibody; GSHB_HUMAN antibody; GSHS antibody; GSS antibody; HEL-S-64p antibody; HEL-S-88n antibody; MGC14098 antibody; OTTHUMP00000030711 antibody

SEQUENCE SIMILARITIES

Belongs to the eukaryotic GSH synthase family.

SUBCELLULAR LOCATION

Cytosol. Extracellular region or secreted.

FUNCTION

GSS (Glutathione synthetase) is a 474 amino acid protein encoded by the gene located at human chromosome 20q11.2. GSS consists of three loops projecting from an antiparallel β-sheet, a parallel β-sheet and a lid of anti-parallel sheets, which provide access to the ATP-binding site. Although Southern blot and gene analysis suggest that GSS may be the only member of a unique family, the crystal structure indicates that GSS belongs to the ATP-GRASP superfamily. GSS is expressed in hemocytes and nucleated cells, including the brain. GSS occurs as a homodimer. There are two steps in the production of Glutathione, begining with γ-GCS and ending with GSS. In an ATP-dependent reaction, GSS produces Glutathione from γ-glutamylcysteine and glycine precursors. Partial hepatectomy, diethyl maleate, buthionine sulfoximine, tert-butylhaydroquinone and thioacetamide increase the ex-pression of GSS, which causes an increase in Glutathione levels. An inherited autosomal recessive disorder, 5-oxoprolinuria (pyroglutamic aciduria), is caused by GSS deficiencies, which leads to central nervous system damage, hemolytic anemia, metabolic acidosis and urinary excretion of 5-oxoproline.