Positive control:
Lane 1: THP-1
Lane 2: HepG2
Lane 3: 293T
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Glutathione Peroxidase 1 Mouse Monoclonal Antibody [C5-F6] (EM40708)
Immunogen
Recombinant protein within human glutathione peroxidase 1 aa 25-203 / 203.
Host
Mouse
Positive Control
Hela, HepG2, 293T, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
C5-F6
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
22 kDa
Isotype
IgG2b
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:200
-
ICC
-
1:200
-
FC
-
1:100-1:200
TARGET
UNIPROT #
PROTEIN NAME
Glutathione Peroxidase 1
SYNONYMS
AL033363 antibody; Cellular glutathione peroxidase antibody; Glutathione peroxidase 1 antibody; Glutathione peroxidase antibody; GPx 1 antibody; GPx-1 antibody; GPX1 antibody; GPX1_HUMAN antibody; GPXD antibody; GSHPx-1 antibody; GSHPX1 antibody; MGC14399 antibody; MGC88245 antibody
SEQUENCE SIMILARITIES
Belongs to the glutathione peroxidase family.
TISSUE SPECIFICITY
Expressed in platelets (at protein level).
POST-TRANSLATIONAL MODIFICATION
During periods of oxidative stress, Sec-49 may react with a superoxide radical, irreversibly lose hydroselenide and be converted to dehydroalanine.
SUBCELLULAR LOCATION
Cytoplasm.
FUNCTION
GPX1 is ubiquitously expressed in many tissues, where it protects cells from oxidative stress. Within cells, it localizes to the cytoplasm and mitochondria. As a glutathione peroxidase, GPx1 functions in the detoxification of hydrogen peroxide, specifically by catalyzing the reduction of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the reduction of other organic hydroperoxides, such as lipid peroxides, to the corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant, but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein encoded by this gene protects from CD95-induced apoptosis in cultured breast cancer cells and inhibits 5-lipoxygenase in blood cells, and its overexpression delays endothelial cell death and increases resistance to toxic challenges, especially oxidative stress. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by the nonsense (stop) codon TGA.