Positive control:
Lane 1: SK-Br-3 cell lysate
Lane 2: 293 cell lysate
Applications
-
WB
-
IHC-P
-
FC
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Glutamine Synthetase Mouse Monoclonal Antibody [A3G2] (EM1902-39)
Immunogen
Recombinant protein within human glutamine synthetase aa 190-373.
Host
Mouse
Positive Control
SK-Br-3 cell lysate, 293 cell lysate, human liver tissue, mouse liver tissue, THP-1.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
A3G2
PROPERTIES
Form
Liquid
Storage Condition
Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G affinity purified.
MOLECULAR WEIGHT
42 kDa
Isotype
IgG2a
APPLICATION DILUTION
-
WB
-
1:500-1:2,000
-
IHC-P
-
1:50-1:200
-
FC
-
1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Glutamine Synthetase
SYNONYMS
cell proliferation-inducing protein 59 antibody; Cgl2214 antibody; GLNA antibody; GLNA_HUMAN antibody; GLNS antibody; GLUL antibody; Glutamate ammonia ligase antibody; Glutamate decarboxylase antibody; Glutamate--ammonia ligase antibody; glutamine synthase antibody; Glutamine synthetase antibody; glutamine synthetase I antibody; GS antibody; PIG 43 antibody; PIG 59 antibody; PIG43 antibody; PIG59 antibody; Proliferation inducing protein 43 antibody
SUBCELLULAR LOCATION
Microsome, Cytosol, Mitochondrion, Cell membrane.
FUNCTION
The protein encoded by this gene belongs to the glutamine synthetase family. It catalyzes the synthesis of glutamine from glutamate and ammonia in an ATP-dependent reaction. This protein plays a role in ammonia and glutamate detoxification, acid-base homeostasis, cell signaling, and cell proliferation. Glutamine is an abundant amino acid, and is important to the biosynthesis of several amino acids, pyrimidines, and purines. Mutations in this gene are associated with congenital glutamine deficiency, and overexpression of this gene was observed in some primary liver cancer samples. There are six pseudogenes of this gene found on chromosomes 2, 5, 9, 11, and 12. Alternative splicing results in multiple transcript variants.