PRODUCT CODE: ET1701-34

GLUT2 Recombinant Rabbit Monoclonal Antibody [JJ20-21] (ET1701-34)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of GLUT2 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of GLUT2 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GLUT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GLUT2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1701-34, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of GLUT2 was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1701-34, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of GLUT2 on HepG2 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1701-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GLUT2 Recombinant Rabbit Monoclonal Antibody [JJ20-21] (ET1701-34)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7, Hela, HepG2, human liver tissue, human pancreas tissue, human kideny tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JJ20-21

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

57 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • FC

  • 1:50-1:100

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

GLUT2

SYNONYMS

liver antibody; Glucose Transporter 2 antibody; Glucose Transporter GLUT2 antibody; Glucose transporter type 2 antibody; Glucose transporter type 2 liver antibody; Glucose transporter, liver/islet antibody; GLUT-2 antibody; GLUT2 antibody; GTR2_HUMAN antibody; GTT2 antibody; SLC2A2 antibody; Solute carrier family 2 (facilitated glucose transporter) member 2 antibody; Solute carrier family 2 facilitated glucose transporter member 2 antibody; Solute carrier family 2, facilitated glucose transporter member 2 antibody

SEQUENCE SIMILARITIES

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.

TISSUE SPECIFICITY

Liver, insulin-producing beta cell, small intestine and kidney.

POST-TRANSLATIONAL MODIFICATION

N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.

SUBCELLULAR LOCATION

Membrane.

FUNCTION

Glucose is fundamental to the metabolism of mammalian cells. Its passage across cell membranes is mediated by a family of transporters termed glucose transporters or Gluts. Glut1, Glut3 and Glut4 are high-affinity transporters, whereas Glut2 is a low-affinity transporter. In adipose and muscle tissue, insulin stimulates a rapid and dramatic increase in glucose uptake, which is largely due to the redistribution of the insulin-inducible glucose transporter Glut4. In response to insulin, Glut4 is quickly shuttled from an intracellular storage site to the plasma membrane, where it binds glucose. In contrast, the ubiquitously expressed glucose transporter Glut1 is constitutively targeted to the plasma membrane and shows a much less dramatic translocation in response to insulin. Glut2 expression is seen in pancreatic beta cells, hepatocytes and basolateral membranes of intestinal and epithelial cells, while the highest expression of Glut3 has been found in neuronal tissue.