GLUT2 Recombinant Rabbit Monoclonal Antibody [JJ20-21] (ET1701-34)
Catalog# ET1701-34
Rabbit Monoclonal to GLUT2
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WB
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IHC-P
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FC
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Human
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Mouse
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Rat
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: PC-12 cell lysate (20 µg/Lane)
Lane 3: Mouse liver tissue lysate (40 µg/Lane)
Lane 4: Mouse small intestine tissue lysate (40 µg/Lane)
Predicted band size: 57 kDa
Observed band size: 50 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-34) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
GLUT2 Recombinant Rabbit Monoclonal Antibody [JJ20-21] (ET1701-34)
Immunogen
Synthetic peptide within human glucose transporter glut2 aa 35-84 / 524.
Host
Rabbit
Positive Control
A549 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse small intestine tissue lysate, human liver tissue, human kidney tissue, human pancreas tissue, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ20-21
RRID
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:50-1:200
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FC
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1:50-1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
GLUT2
SYNONYMS
liver antibody;Glucose Transporter 2 antibody;Glucose Transporter GLUT2 antibody;Glucose transporter type 2 antibody;Glucose transporter type 2 liver antibody;Glucose transporter, liver/islet antibody;GLUT-2 antibody;GLUT2 antibody;GTR2_HUMAN antibody;GTT2 antibody;SLC2A2 antibody;Solute carrier family 2 (facilitated glucose transporter) member 2 antibody;Solute carrier family 2 facilitated glucose transporter member 2 antibody;Solute carrier family 2, facilitated glucose transporter member 2 antibody
SEQUENCE SIMILARITIES
Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
TISSUE SPECIFICITY
Liver, insulin-producing beta cell, small intestine and kidney.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated; required for stability and retention at the cell surface of pancreatic beta cells.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Glucose transporter 2 (GLUT2) also known as solute carrier family 2 (facilitated glucose transporter), member 2 (SLC2A2) is a transmembrane carrier protein that enables protein facilitated glucose movement across cell membranes. It is the principal transporter for transfer of glucose between liver and blood. Unlike GLUT4, it does not rely on insulin for facilitated diffusion. GLUT2 has high capacity for glucose but low affinity (high KM, ca. 15–20 mM) and thus functions as part of the "glucose sensor" in the pancreatic β-cells of rodents, though in human β-cells the role of GLUT2 seems to be a minor one. It is a very efficient carrier for glucose. GLUT2 also carries glucosamine. When the glucose concentration in the lumen of the small intestine goes above 30 mM, such as occurs in the fed-state, GLUT2 is up-regulated at the brush border membrane, enhancing the capacity of glucose transport. Basolateral GLUT2 in enterocytes also aids in the transport of fructose into the bloodstream through glucose-dependent cotransport.
CITATIONS
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Chen Zhenzhen,et al
Effects of Three Kinds of Carbohydrate Pharmaceutical Excipients-Fructose, Lactose and Arabic Gum on Intestinal Absorption of Gastrodin through Glucose Transport Pathway in Rats
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WB
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Rat
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Citation
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