PRODUCT CODE: ER1802-96

GLUR Rabbit Polyclonal Antibody (ER1802-96)

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution.<br />
 Positive control:<br />
 Lane 1: A549 <br />
    Lane 2: Rat liver tissue
  • Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution.<br />
 Positive control:<br />
 Lane 1: A549 <br />
    Lane 2: Rat liver tissue
  • ICC staining GLUR in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GLUR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GLUR antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-GLUR antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with GLUR antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of GLUR on different lysates using anti-GLUR antibody at 1/2,000 dilution.
Positive control:
Lane 1: A549
Lane 2: Rat liver tissue

Applications

  • WB

  • ICC

  • IHC-P

  • FC

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

GLUR Rabbit Polyclonal Antibody (ER1802-96)

Immunogen

Recombinant protein within human glur aa 273-446 / 522.

Host

Rabbit

Positive Control

A549, rat liver tissue, rat epididymis tissue, human liver tissue, human thyroid gland tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

56 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:2,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

GLUR

SYNONYMS

GLR1 antibody; GLUR antibody; Glutathione reductase antibody; Glutathione reductase mitochondrial antibody; Glutathione reductase, mitochondrial antibody; GR antibody; Gr1 antibody; GRase antibody; GRD 1 antibody; GRD1 antibody; GSHR_HUMAN antibody; GSR antibody; MGC78522 antibody

SEQUENCE SIMILARITIES

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

SUBCELLULAR LOCATION

Cytoplasm. Mitochondrion.

FUNCTION

Glutathione plays a key role in maintaining proper function and preventing oxidative stress in human cells. It can act as a scavenger for hydroxyl radicals, singlet oxygen, and various electrophiles. Reduced glutathione reduces the oxidized form of the enzyme glutathione peroxidase, which in turn reduces hydrogen peroxide (H2O2), a dangerously reactive species within the cell. In addition, it plays a key role in the metabolism and clearance of xenobiotics, acts as a cofactor in certain detoxifying enzymes, participates in transport, and regenerates antioxidants such and Vitamins E and C to their reactive forms. The ratio of GSSG/GSH present in the cell is a key factor in properly maintaining the oxidative balance of the cell, that is, it is critical that the cell maintains high levels of the reduced glutathione and a low level of the oxidized Glutathione disulfide. This narrow balance is maintained by glutathione reductase, which catalyzes the reduction of GSSG to GSH.