PRODUCT CODE: ET7108-01

Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody [JG33-86] (ET7108-01)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

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Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.
  • Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.
  • ICC staining Glucose 6 phosphate isomerase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Glucose 6 phosphate isomerase in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Glucose 6 phosphate isomerase antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of A549 cells with Glucose 6 phosphate isomerase antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Glucose 6 phosphate isomerase on A549 and HepG2 cells lysates using anti-Glucose 6 phosphate isomerase antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Glucose 6 phosphate isomerase Recombinant Rabbit Monoclonal Antibody [JG33-86] (ET7108-01)

Immunogen

Recombinant protein corresponding to human glucose 6 phosphate isomerase 300-500aa.

Host

Rabbit

Positive Control

A549, HepG2, LO2, MCF-7, human colon tissue, human prostate tissue, human kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JG33-86

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

63 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

Glucose 6 phosphate isomerase

SYNONYMS

AMF antibody; Aurocrine motility factor antibody; Autocrine motility factor antibody; DKFZp686C13233 antibody; EC 5.3.1.9 antibody; G6PI_HUMAN antibody; Glucose phosphate isomerase antibody; Glucose-6-phosphate isomerase antibody; GNPI antibody; GPI antibody; Gpi1 antibody; Hexose monophosphate isomerase antibody; Hexosephosphate isomerase antibody; Neuroleukin antibody; NLK antibody; Oxoisomerase antibody; PGI antibody; PHI antibody; Phosphoglucose isomerase antibody; Phosphohexomutase antibody; Phosphohexose isomerase antibody; Phosphosaccharomutase antibody; SA 36 antibody; SA-36 antibody; SA36 antibody

SEQUENCE SIMILARITIES

Belongs to the GPI family.

POST-TRANSLATIONAL MODIFICATION

Phosphorylation at Ser-185 by CK2 has been shown to decrease enzymatic activity and may contribute to secretion by a non-classical secretory pathway.; ISGylated.

SUBCELLULAR LOCATION

Cytoplasm. Secreted.

FUNCTION

Glucose-6-phosphate isomerase (GPI) has many other names, including Phosphohexose isomerase (PHI), Neuroleukin (NLK) and Spermantigen-36 (SA-36). GPI is a cytoplasmic homodimer belonging to the GPI family. It is a neurotrophic factor for spinal and sensory neurons and is involved in glycolysis and gluconeogenesis. Defects or mutations in GPI can cause hereditary nonspherocytic hemolytic anemia (HA), hydrops fetalis, immediate neonatal death and neurological impairment.