GLP1 Recombinant Rabbit Monoclonal Antibody [JF33-10] (ET1702-59)

ICC staining GLP1 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Applications

WB
ICC
IF
IHC-P

REACTIVITY

Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

GLP1 Recombinant Rabbit Monoclonal Antibody [JF33-10] (ET1702-59)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

PC-12, SW480, human pancreas tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JF33-10

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

21/8/4 kDa

Isotype

IgG

APPLICATION DILUTION

500-1:1,000

ICC/IF
1:50-1:200

IHC-P
1:50-1:200

TARGET

UNIPROT #

P01275

PROTEIN NAME

GLP1

SYNONYMS

GCG antibody; glicentin-related polypeptide antibody; GLP-1 antibody; GLP-1(7-36) antibody; GLP-1(7-37) antibody; GLP-2 antibody; GLP1 antibody; GLP1, included antibody; GLP2 antibody; GLP2, included antibody; GLUC_HUMAN antibody; Glucagon antibody; Glucagon like peptide 1 antibody; glucagon-like peptide 1 antibody; Glucagon-like peptide 1, included antibody; Glucagon-like peptide 2 antibody; Glucagon-like peptide 2, included antibody; GRPP antibody; OXM antibody; OXY antibody; preproglucagon antibody

SEQUENCE SIMILARITIES

Belongs to the glucagon family.

TISSUE SPECIFICITY

Glucagon is secreted in the A cells of the islets of Langerhans. GLP-1, GLP-2, oxyntomodulin and glicentin are secreted from enteroendocrine cells throughout the gastrointestinal tract. GLP-1 and GLP-2 are also secreted in selected neurons in the brain.

POST-TRANSLATIONAL MODIFICATION

Proglucagon is post-translationally processed in a tissue-specific manner in pancreatic A cells and intestinal L cells. In pancreatic A cells, the major bioactive hormone is glucagon cleaved by PCSK2/PC2. In the intestinal L cells PCSK1/PC1 liberates GLP-1, GLP-2, glicentin and oxyntomodulin. GLP-1 is further N-terminally truncated by post-translational processing in the intestinal L cells resulting in GLP-1(7-37) GLP-1-(7-36)amide. The C-terminal amidation is neither important for the metabolism of GLP-1 nor for its effects on the endocrine pancreas.

SUBCELLULAR LOCATION

Secreted.

FUNCTION

Glucagon is a pancreatic hormone that functions as an antagonist to insulin, stimulating the conversion of glycogen to glucose and increasing blood sugar levels. Glucagon-like peptide-1 (GLP-1), Glucagon-like peptide-2 (GLP-2), VIP (vasoactive intestinal peptide) and PACAP (pituitary adenylate cyclase activating polypeptide) are members of the Glucagon family of hormones. GLP-1 functions as a transmitter in the central nervous system, inhibiting feeding and drinking behavior, whereas GLP-2 is a stimulator of intestinal epithelial growth. VIP causes vasodilation resulting in the lowering of blood pressure. PACAP is abundant in the hypothalamus and has been shown to increase the synthesis of several hormones, including growth hormone.